Cryogenically Structured Extracellular Matrix Mimetic Based on a Concentrated Collagen-Containing Solution

IF 0.5 Q4 MATERIALS SCIENCE, MULTIDISCIPLINARY Inorganic Materials: Applied Research Pub Date : 2024-05-27 DOI:10.1134/s2075113324020096

Yu. B. Basok, A. M. Grigoriev, V. I. Lozinsky, L. A. Kirsanova, V. K. Kulakova, E. A. Podorozhko, I. A. Novikov, V. I. Sevastianov

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Abstract

A new macroporous cryogenically structured biomimetic of the extracellular matrix (ECM) was prepared on the basis of a commercially available concentrated collagen-containing solution, and the possibility of its use in tissue engineering was assessed. Spongy collagen-containing material was fabricated by sequential freezing of a concentrated collagen-containing solution, its subsequent lyophilization, followed by chemical tanning by treatment with an alcohol solution of carbodiimide. The morphology of the cryostructured multicomponent collagen-containing material was studied by optical and scanning electron microscopy (SEM) using lanthanide contrast. The cytotoxicity of the matrix was studied in a culture of human adipose tissue-derived mesenchymal stromal cells (hADSCs). Adhesion and proliferation of hADSCs on the surface of the matrix were studied on the seventh day of cultivation. The compression modulus of elasticity of the resulting collagen-containing material in a water-swollen state was 35.3 ± 2.2 kPa, the total water-holding capacity of the material was 45.80 ± 0.46 mL/g of polymer, and the degree of swelling of the macropore walls was 3.99 ± 0.31 mL/g. During SEM examination and histological staining with hematoxylin and eosin, a large-pored structure was observed on the surface and cross section of the disk. The pores in the upper part are larger (average diameter at least ~30 µm) than the pores in the lower part of the sponge (average diameter at most ~30 µm) owing to the occurrence of a vertical temperature gradient. The matrix did not have cytotoxicity toward hADSCs. In the sample, active proliferation of hADSCs was observed on the surface of the matrix. The lack of cytotoxicity and the ability to support the adhesion and proliferation of hADSCs indicate the possibility of using cryogenically structured extracellular matrix biomimetic in tissue engineering and regenerative medicine.

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基于浓缩胶原蛋白溶液的低温结构细胞外基质模拟物

摘要在市售浓缩胶原蛋白溶液的基础上制备了一种新的大孔低温结构仿细胞外基质（ECM）生物材料，并对其在组织工程中的应用可能性进行了评估。含海绵状胶原蛋白的材料是通过连续冷冻含胶原蛋白的浓缩溶液，然后冻干，再用碳化二亚胺的酒精溶液进行化学鞣制而制成的。利用镧系元素对比，通过光学和扫描电子显微镜（SEM）研究了冷冻结构多组分胶原蛋白材料的形态。在人脂肪组织间充质基质细胞（hADSCs）培养中研究了基质的细胞毒性。在培养的第七天，研究了 hADSCs 在基质表面的粘附和增殖情况。所得含胶原蛋白材料在水膨胀状态下的压缩弹性模量为 35.3 ± 2.2 kPa，材料的总持水量为 45.80 ± 0.46 mL/g（聚合物），大孔壁的膨胀度为 3.99 ± 0.31 mL/g。通过扫描电子显微镜检查和苏木精及伊红组织学染色，可以在圆盘的表面和横截面上观察到大孔隙结构。由于存在垂直温度梯度，海绵上部的孔隙（平均直径至少约 30 微米）比海绵下部的孔隙（平均直径最多约 30 微米）大。基质对 hADSCs 没有细胞毒性。在样品中，可以观察到 hADSCs 在基质表面活跃增殖。低温结构细胞外基质没有细胞毒性，而且能够支持 hADSCs 的粘附和增殖，这表明低温结构细胞外基质生物仿生材料有可能用于组织工程和再生医学。

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来源期刊

Inorganic Materials: Applied Research Engineering-Engineering (all)

CiteScore

0.90

自引率

0.00%

发文量

199

期刊介绍： Inorganic Materials: Applied Research contains translations of research articles devoted to applied aspects of inorganic materials. Best articles are selected from four Russian periodicals: Materialovedenie, Perspektivnye Materialy, Fizika i Khimiya Obrabotki Materialov, and Voprosy Materialovedeniya and translated into English. The journal reports recent achievements in materials science: physical and chemical bases of materials science; effects of synergism in composite materials; computer simulations; creation of new materials (including carbon-based materials and ceramics, semiconductors, superconductors, composite materials, polymers, materials for nuclear engineering, materials for aircraft and space engineering, materials for quantum electronics, materials for electronics and optoelectronics, materials for nuclear and thermonuclear power engineering, radiation-hardened materials, materials for use in medicine, etc.); analytical techniques; structure–property relationships; nanostructures and nanotechnologies; advanced technologies; use of hydrogen in structural materials; and economic and environmental issues. The journal also considers engineering issues of materials processing with plasma, high-gradient crystallization, laser technology, and ultrasonic technology. Currently the journal does not accept direct submissions, but submissions to one of the source journals is possible.