{"title":"miR-885-5p Predicts the Risk and Development of Intracranial Hemorrhage in Traumatic Brain Injury and Regulates Inflammation in Microglia","authors":"Litao Shi, Rong Yang, Yaqian Wang","doi":"10.1134/s1819712424020132","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Intracranial hemorrhage (ICH) is the most critical secondary lesion of traumatic brain injury (TBI). Identifying novel biomarkers for the prediction of ICH occurrence and development in TBI patients would benefit the prognosis of TBI and provide targeted nursing strategies for ICH secondary to TBI. The study enrolled a total of 208 TBI patients, where 105 patients had ICH. Serum samples were collected and analyzed with PCR to evaluate the expression of miR-885-5p. The significance of miR-885-5p in predicting the risk and progression of ICH in TBI patients was assessed. Microglia was induced by lipopolysaccharide (LPS) and transfected with miR-885-5p mimic. The inflammation in microglia was estimated by the levels of TNF-α, IL-6, and IL-1β using ELISA. Significant downregulation of miR-885-5p was observed in ICH patients secondary to TBI, which was identified as a risk factor for ICH and discriminated TBI-ICH patients from TBI patients without secondary lesions. Reduced serum miR-885-5p was significantly associated with lower GCS score, lower NIHSS score, increasing intracranial hemorrhage, increasing edema volume of peripheral tissues, and dysregulated coagulation function of ICH patients. In microglia, LPS induced the downregulation of miR-885-5p and increasing levels of TNF-α, IL-6, and IL-1β. The overexpression of miR-885-5p could alleviate LPS-induced inflammation in microglia. Downregulated miR-885-5p predicted the occurrence and severity of ICH secondary to TBI and regulated neuroinflammation in microglia.</p>","PeriodicalId":19119,"journal":{"name":"Neurochemical Journal","volume":"62 1","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neurochemical Journal","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1134/s1819712424020132","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Intracranial hemorrhage (ICH) is the most critical secondary lesion of traumatic brain injury (TBI). Identifying novel biomarkers for the prediction of ICH occurrence and development in TBI patients would benefit the prognosis of TBI and provide targeted nursing strategies for ICH secondary to TBI. The study enrolled a total of 208 TBI patients, where 105 patients had ICH. Serum samples were collected and analyzed with PCR to evaluate the expression of miR-885-5p. The significance of miR-885-5p in predicting the risk and progression of ICH in TBI patients was assessed. Microglia was induced by lipopolysaccharide (LPS) and transfected with miR-885-5p mimic. The inflammation in microglia was estimated by the levels of TNF-α, IL-6, and IL-1β using ELISA. Significant downregulation of miR-885-5p was observed in ICH patients secondary to TBI, which was identified as a risk factor for ICH and discriminated TBI-ICH patients from TBI patients without secondary lesions. Reduced serum miR-885-5p was significantly associated with lower GCS score, lower NIHSS score, increasing intracranial hemorrhage, increasing edema volume of peripheral tissues, and dysregulated coagulation function of ICH patients. In microglia, LPS induced the downregulation of miR-885-5p and increasing levels of TNF-α, IL-6, and IL-1β. The overexpression of miR-885-5p could alleviate LPS-induced inflammation in microglia. Downregulated miR-885-5p predicted the occurrence and severity of ICH secondary to TBI and regulated neuroinflammation in microglia.
期刊介绍：
Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components.
The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.
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