Enhanced anticancer activity of (-)-epigallocatechin-3-gallate (EGCG) encapsulated NPs toward colon cancer cell lines.

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Free Radical Research Pub Date : 2024-10-01 Epub Date: 2024-06-22 DOI:10.1080/10715762.2024.2360013
Tanushree Das, Sanchaita Mondal, Sujata Das, Sanjib Das, Krishna Das Saha
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Abstract

(-)-Epigallocatechin-3-gallate (EGCG), a bioactive polyphenol of green tea, has chemo-preventive effects against various cancer cells. Nanoparticles (NPs) carrying different ligands are able to specifically interact with their receptors on different cancer cells that can provide effective release of cytotoxic drugs. In the present study, we have prepared EGCG entrapped NPs using PLGA (poly(d,l-lactide-co-glycolide)). Polyethylene glycol (PEG) and folic acid (FA) via double emulsion solvent evaporation (DESE) method obtained PLGA-EGCG (P-E), PLGA-PEG-EGCG (PP-E), and PLGA-PEG-FA-EGCG (PPF-E). Nanoformulations had been characterized with 1H NMR and FT-IR techniques, AFM, and DLS. PPF-E NPs showed an average size of 220 nm. Analysis of zeta potential confirmed the stability of NPs. HCT-116, HT-29, HCT-15, and HEK 293 cells were treated with both the prepared NPs and free EGCG (0-140 μM). Result showed PPF-E NPs had improved delivery, uptake and cell cytotoxicity toward human folic acid receptor-positive (FR+) colorectal cancer (CRC) cells as mainly on HCT-116 compared to HT-29, but not on the folic acid-negative cells (FR-) as HCT-15. PPF-E NPs enhanced intracellular reactive oxygen species (ROS) level in absence of N-acetyl-l-cysteine (NAC), elevated DNA fragmentation level, and increased apoptotic cell death at higher doses compared to other two NPs and free EGCG. In conclusion, PPF-E NPs exerted greater efficacy than PP-E, P-E, and free EGCG in HCT-116 cells.

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增强(-)-表没食子儿茶素-3-棓酸盐(EGCG)包裹的 NPs 对结肠癌细胞株的抗癌活性。
(-)-表没食子儿茶素-3-棓酸盐(EGCG)是绿茶中的一种生物活性多酚,对多种癌细胞具有化学预防作用。携带不同配体的纳米粒子(NPs)能够与不同癌细胞上的受体发生特异性相互作用,从而有效释放细胞毒性药物。在本研究中,我们使用 PLGA(聚乳酸-聚乙二醇)制备了夹带 EGCG 的纳米颗粒。采用 1H NMR、FT-IR、AFM 和 DLS 对制备的 PLGA-EGCG (P-E)、PLGA-PEG-EGCG (PP-E) 和 PLGA-PEG-FA-EGCG (PPF-E) 纳米制剂进行了表征。PPF-E NPs 的平均尺寸为 220 纳米。Zeta 电位分析证实了 NPs 的稳定性。用制备的 NPs 和游离 EGCG(0-140μM)处理 HCT 116、HT-29、HCT-15 和 HEK 293 细胞。结果表明,与 HT-29 相比,PPF-E NPs 对人类叶酸受体阳性(FR+)结直肠癌细胞(CRCs)的递送、吸收和细胞毒性都有所改善,主要是在 HCT116 上,但在叶酸阴性(FR-)细胞(如 HCT-15)上则没有改善。与其他两种NPs和游离的EGCG相比,PPF-E NPs在没有NAC(N-乙酰-L-半胱氨酸)的情况下会提高细胞内活性氧(ROS)的水平,升高DNA碎片水平,并在更高剂量下增加细胞凋亡。总之,在 HCT 116 细胞中,PPF-E NPs 比 PP-E、P-E 和游离的 EGCG 具有更强的功效。
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来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
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