Electroacupuncture treatment improves postoperative ileus by inhibiting the Th1 cell-mediated inflammatory response through the vagus nerve.

IF 2.4 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Acupuncture in Medicine Pub Date : 2024-06-01 Epub Date: 2024-05-30 DOI:10.1177/09645284241248466

Hao Gao, Liyue Lu, Lili Li, Zhiyu Yin, Wenqing Ruan, Yinghua Zou, He Li, Yalan Zhou, Xing Li, Yongqiang Wang, Jiangang Song, Wenting Chen, Jian Wang

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Abstract

Background: Electroacupuncture (EA) has been reported to improve intestinal motility in mice with postoperative ileus (POI). Previous studies, however, have yielded heterogeneous results regarding the effect of EA on POI.

Methods: Herein, a POI mouse model was constructed by intestinal manipulation. To evaluate the effect of EA treatment on colonic transit, the levels of inflammatory markers (macrophage inflammatory protein (MIP)-1α, interleukin (IL)-1β, IL-6, monocyte chemotactic protein (MCP)-1 and intercellular adhesion molecule (ICAM)-1) were detected by enzyme-linked immunosorbent assay (ELISA); immune cell infiltration was detected by immunohistochemical staining of myeloperoxidase (MPO), ectodysplasin (ED)-1 and ED-2, and the percentage of CD4⁺ interferon (IFN)-γ⁺ Th1 cells and IFN-γ secretion levels were determined. Activated Th1 cells and pentoxifylline, a cell differentiation inhibitor, were used to assess the role of Th1 cells in EA treatment of POI. Neostigmine administration and unilateral vagotomy were performed to confirm whether the effects of EA treatment on Th1 cells were mediated by the vagus nerve (VN).

Results: The results revealed that EA treatment at ST36 improved POI, as indicated by a decreased level of inflammatory-related markers and immune cell infiltration and shortened colonic transit time. The activated Th1 cells abolished the effects of EA treatment on POI. The effects of EA treatment on POI were enhanced by stimulation of the VN along with a decreased level of Th1 cells, but these effects were abolished by vagotomy along with an increased percentage of Th1 cells; this result indicates that the VN mediates the role of Th1 cells in the effects of EA treatment of POI.

Conclusion: Our findings showed that the effects of EA treatment of POI were mainly mediated by Th1 cells through the stimulation of the VN and inhibition of the inflammatory response.

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电针治疗通过迷走神经抑制 Th1 细胞介导的炎症反应，从而改善术后回肠梗阻。

背景：有报道称电针（EA）可改善术后回肠梗阻（POI）小鼠的肠蠕动。方法：本文通过肠道操作建立了 POI 小鼠模型。为了评估 EA 治疗对结肠转运的影响，用酶联免疫吸附试验（ELISA）检测了炎症标志物（巨噬细胞炎症蛋白（MIP）-1α、白细胞介素（IL）-1β、IL-6、单核细胞趋化蛋白（MCP）-1 和细胞间粘附分子（ICAM）-1）的水平；免疫组织化学染色法检测髓过氧化物酶（MPO）、外胚层蛋白（ED）-1 和 ED-2，并测定 CD4+ 干扰素（IFN）-γ+ Th1 细胞的百分比和 IFN-γ 的分泌水平。活化的 Th1 细胞和细胞分化抑制剂喷托非林被用来评估 Th1 细胞在 EA 治疗 POI 中的作用。为了证实 EA 治疗对 Th1 细胞的影响是否由迷走神经（VN）介导，还进行了新斯的明给药和单侧迷走神经切断术：结果：结果表明，在 ST36 段进行 EA 治疗可改善 POI，这表现在炎症相关标记物和免疫细胞浸润水平降低以及结肠转运时间缩短。活化的 Th1 细胞消除了 EA 处理对 POI 的影响。刺激迷走神经可增强 EA 治疗对 POI 的影响，同时降低 Th1 细胞的水平，但迷走神经切断可消除这些影响，同时增加 Th1 细胞的比例；这一结果表明迷走神经在 EA 治疗对 POI 的影响中介导了 Th1 细胞的作用：我们的研究结果表明，EA 治疗 POI 的效果主要由 Th1 细胞通过刺激 VN 和抑制炎症反应介导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.