The activation of AMPK/PGC-1α/GLUT4 signaling pathway through early exercise improves mitochondrial function and mitigates ischemic brain damage.

IF 1.6 4区 医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-07-01 Epub Date: 2024-05-15 DOI:10.1097/WNR.0000000000002048
Xinyue Wu, Chen Li, Changkai Ke, Chuan Huang, Bingchen Pan, Chunxiao Wan
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Abstract

Mitochondria play a crucial role in maintaining cellular energy supply and serve as a source of energy for repairing nerve damage following a stroke. Given that exercise has the potential to enhance energy metabolism, investigating the impact of exercise on mitochondrial function provides a plausible mechanism for stroke treatment. In our study, we established the middle cerebral artery occlusion (MCAO) model in Sprague-Dawley rats and implemented early exercise intervention. Neurological severity scores, beam-walking test score, and weight were used to evaluate neurological function. The volume of cerebral infarction was measured by MRI. Nerve cell apoptosis was detected by TUNEL staining. Mitochondrial morphology and structure were detected by mitochondrial electron microscopy. Mitochondrial function was assessed using membrane potential and ATP measurements. Western blotting was used to detect the protein expression of AMPK/PGC-1α/GLUT4. Through the above experiments, we found that early exercise improved neurological function in rats after MCAO, reduced cerebral infarction volume and neuronal apoptosis, promoted the recovery of mitochondrial morphology and function. We further examined the protein expression of AMPK/PGC-1α/GLUT4 signaling pathway and confirmed that early exercise was able to increase its expression. Therefore, we suggest that early exercise initiated the AMPK/PGC-1α/GLUT4 signaling pathway, restoring mitochondrial function and augmenting energy supply. This, in turn, effectively improved both nerve and body function in rats following ischemic stroke.

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通过早期运动激活 AMPK/PGC-1α/GLUT4 信号通路,可改善线粒体功能,减轻缺血性脑损伤。
线粒体在维持细胞能量供应方面起着至关重要的作用,是中风后修复神经损伤的能量来源。鉴于运动具有增强能量代谢的潜力,研究运动对线粒体功能的影响为中风治疗提供了一种可信的机制。在我们的研究中,我们在 Sprague-Dawley 大鼠中建立了大脑中动脉闭塞(MCAO)模型,并实施了早期运动干预。神经严重程度评分、横梁行走测试评分和体重用于评估神经功能。核磁共振成像测量了脑梗塞的体积。通过 TUNEL 染色检测神经细胞凋亡。线粒体电子显微镜检测线粒体形态和结构。线粒体功能通过膜电位和 ATP 测量进行评估。用 Western 印迹法检测 AMPK/PGC-1α/GLUT4 的蛋白表达。通过以上实验,我们发现早期运动能改善 MCAO 后大鼠的神经功能,减少脑梗死体积和神经元凋亡,促进线粒体形态和功能的恢复。我们进一步检测了AMPK/PGC-1α/GLUT4信号通路的蛋白表达,证实早期运动能够增加其表达。因此,我们认为早期运动启动了AMPK/PGC-1α/GLUT4信号通路,恢复了线粒体功能并增加了能量供应。这反过来又有效改善了缺血性中风后大鼠的神经和身体功能。
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来源期刊
Neuroreport
Neuroreport 医学-神经科学
CiteScore
3.20
自引率
0.00%
发文量
150
审稿时长
1 months
期刊介绍: NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.
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