Advancing skeletal health and disease research with single-cell RNA sequencing.

IF 16.7 2区 医学 Q1 MEDICINE, GENERAL & INTERNAL Military Medical Research Pub Date : 2024-05-30 DOI:10.1186/s40779-024-00538-3
Peng Lin, Yi-Bo Gan, Jian He, Si-En Lin, Jian-Kun Xu, Liang Chang, Li-Ming Zhao, Jun Zhu, Liang Zhang, Sha Huang, Ou Hu, Ying-Bo Wang, Huai-Jian Jin, Yang-Yang Li, Pu-Lin Yan, Lin Chen, Jian-Xin Jiang, Peng Liu
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Abstract

Orthopedic conditions have emerged as global health concerns, impacting approximately 1.7 billion individuals worldwide. However, the limited understanding of the underlying pathological processes at the cellular and molecular level has hindered the development of comprehensive treatment options for these disorders. The advent of single-cell RNA sequencing (scRNA-seq) technology has revolutionized biomedical research by enabling detailed examination of cellular and molecular diversity. Nevertheless, investigating mechanisms at the single-cell level in highly mineralized skeletal tissue poses technical challenges. In this comprehensive review, we present a streamlined approach to obtaining high-quality single cells from skeletal tissue and provide an overview of existing scRNA-seq technologies employed in skeletal studies along with practical bioinformatic analysis pipelines. By utilizing these methodologies, crucial insights into the developmental dynamics, maintenance of homeostasis, and pathological processes involved in spine, joint, bone, muscle, and tendon disorders have been uncovered. Specifically focusing on the joint diseases of degenerative disc disease, osteoarthritis, and rheumatoid arthritis using scRNA-seq has provided novel insights and a more nuanced comprehension. These findings have paved the way for discovering novel therapeutic targets that offer potential benefits to patients suffering from diverse skeletal disorders.

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利用单细胞 RNA 测序推进骨骼健康和疾病研究。
骨科疾病已成为全球关注的健康问题,影响着全球约 17 亿人。然而,由于对细胞和分子水平的潜在病理过程了解有限,阻碍了这些疾病的综合治疗方案的开发。单细胞 RNA 测序(scRNA-seq)技术的出现为生物医学研究带来了革命性的变化,可以对细胞和分子多样性进行详细的检查。然而,在高度矿化的骨骼组织中研究单细胞水平的机制却面临着技术上的挑战。在这篇综述中,我们介绍了从骨骼组织中获取高质量单细胞的简化方法,并概述了骨骼研究中采用的现有 scRNA-seq 技术以及实用的生物信息学分析管道。通过利用这些方法,我们对脊柱、关节、骨骼、肌肉和肌腱疾病所涉及的发育动态、平衡维持和病理过程有了重要的认识。利用 scRNA-seq 对椎间盘退行性病变、骨关节炎和类风湿性关节炎等关节疾病的研究提供了新的见解和更细致的理解。这些发现为发现新的治疗靶点铺平了道路,从而为各种骨骼疾病患者带来潜在的益处。
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来源期刊
Military Medical Research
Military Medical Research Medicine-General Medicine
CiteScore
38.40
自引率
2.80%
发文量
485
审稿时长
8 weeks
期刊介绍: Military Medical Research is an open-access, peer-reviewed journal that aims to share the most up-to-date evidence and innovative discoveries in a wide range of fields, including basic and clinical sciences, translational research, precision medicine, emerging interdisciplinary subjects, and advanced technologies. Our primary focus is on modern military medicine; however, we also encourage submissions from other related areas. This includes, but is not limited to, basic medical research with the potential for translation into practice, as well as clinical research that could impact medical care both in times of warfare and during peacetime military operations.
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