Metabarcoding of protozoa and helminth in black-necked cranes: a high prevalence of parasites and free-living amoebae.

IF 2.3 2区 医学 Q2 PARASITOLOGY Parasite Pub Date : 2024-01-01 Epub Date: 2024-05-30 DOI:10.1051/parasite/2024028
Mengshi Yu, Wenhao Li, Xin He, Guiwen He, Yonfang Yao, Yuanjian Wang, Mingcui Shao, Tingsong Xiong, Huailiang Xu, Junsong Zhao
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Abstract

Parasites and free-living amoebae (FLA) are common pathogens that pose threats to wildlife and humans. The black-necked crane (Grus nigricollis) is a near-threatened species and there is a shortage of research on its parasite diversity. Our study aimed to use noninvasive methods to detect intestinal parasites and pathogenic FLA in G. nigricollis using high-throughput sequencing (HTS) based on the 18S rDNA V9 region. A total of 38 fresh fecal samples were collected in Dashanbao, China, during the overwintering period (early-, middle I-, middle II-, and late-winter). Based on the 18S data, eight genera of parasites were identified, including three protozoan parasites: Eimeria sp. (92.1%) was the dominant parasite, followed by Tetratrichomonas sp. (36.8%) and Theileria sp. (2.6%). Five genera of helminths were found: Echinostoma sp. (100%), Posthodiplostomum sp. (50.0%), Euryhelmis sp. (26.3%), Eucoleus sp. (50.0%), and Halomonhystera sp. (2.6%). Additionally, eight genera of FLA were detected, including the known pathogens Acanthamoeba spp. (n = 13) and Allovahlkampfia spp. (n = 3). Specific PCRs were used to further identify the species of some parasites and FLA. Furthermore, the 18S data indicated significant changes in the relative abundance and genus diversity of the protozoan parasites and FLA among the four periods. These results underscore the importance of long-term monitoring of pathogens in black-necked cranes to protect this near-endangered species.

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黑颈鹤体内原生动物和蠕虫的代谢编码:寄生虫和自由生活变形虫的高流行率。
寄生虫和自由生活阿米巴原虫(FLA)是对野生动物和人类构成威胁的常见病原体。黑颈鹤(Grus nigricollis)是一种濒临灭绝的物种,对其寄生虫多样性的研究十分匮乏。我们的研究旨在使用非侵入性方法,通过基于 18S rDNA V9 区域的高通量测序(HTS)来检测黑颈鹤的肠道寄生虫和致病性 FLA。研究人员在中国大山包采集了 38 份越冬期(初冬、Ⅰ冬中期、Ⅱ冬中期和Ⅲ冬后期)的新鲜粪便样本。根据 18S 数据,确定了 8 个寄生虫属,包括 3 种原生动物寄生虫:最主要的寄生虫是艾美耳属(92.1%),其次是四膜单胞菌属(36.8%)和毛癣菌属(2.6%)。发现了五种蠕虫属:Echinostoma sp.(100%)、Posthodiplostomum sp.(50.0%)、Euryhelmis sp.(26.3%)、Eucoleus sp.(50.0%)和 Halomonhystera sp.(2.6%)。此外,还检测到 8 个 FLA 属,包括已知的病原体 Acanthamoeba spp.(n = 13)和 Allovahlkampfia spp.(n = 3)。利用特异性 PCR 进一步确定了一些寄生虫和 FLA 的种类。此外,18S 数据表明,在四个时期中,原生动物寄生虫和 FLA 的相对丰度和种属多样性发生了显著变化。这些结果强调了长期监测黑颈鹤病原体对保护这一濒危物种的重要性。
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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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