Cyclic AMP phosphodiesterase activities in growing cells of baker's yeast (Saccharomyces cerevisiae).

K Suoranta
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Abstract

The activities of two cyclic AMP phosphodiesterases of baker's yeast (Saccharomyces cerevisiae) were measured during diauxic batch growth on 2% glucose. The specific activity (units/mg of yeast protein) of the Mg-independent, high Km phosphodiesterase increased 20-fold throughout the 108 h cultivation. The specific activity of the Mg-dependent, low Km phosphodiesterase about doubled during glucose utilization and fell back to the initial level as the cells entered stationary phase.

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酵母(Saccharomyces cerevisiae)生长细胞中的环AMP磷酸二酯酶活性。
测定了面包酵母(Saccharomyces cerevisiae)在2%葡萄糖条件下双补批生长过程中两种环AMP磷酸二酯酶的活性。在108 h的培养过程中,与mg无关的高Km磷酸二酯酶的比活性(单位/mg酵母蛋白)提高了20倍。mg依赖性、低Km磷酸二酯酶的比活性在葡萄糖利用过程中增加了一倍左右,并在细胞进入固定期时回落到初始水平。
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