Mice learn to identify and discriminate sugar solutions based on odor cues.

IF 2.8 4区 心理学 Q1 BEHAVIORAL SCIENCES Chemical Senses Pub Date : 2024-01-01 DOI:10.1093/chemse/bjae024
John I Glendinning, Alix Archambeau, Catherine Conlin, Zoee Drimmer, Gabriel Griffith, Rayna Isber, Kayla Koffler, Gabriella Ortiz, Anisha Prakash, Catherine Sollitto, Harini Srinivasan
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Abstract

This study examined how olfaction impacts ingestive responses of mice to sugar solutions. Experiment 1 asked whether naïve C57BL/6 (B6) mice could identify 1 M glucose, fructose, or sucrose solutions based on odor cues, during a 30-min 2-bottle acceptability test. We tested mice both before and after they were rendered anosmic with ZnSO4 treatment. We used 2 indirect measures of odor-mediated response: number of trials initiated and latency to initiate licking. Before ZnSO4 treatment, the mice learned how to identify 1 M glucose and fructose (but not sucrose) solutions based on odor cues. ZnSO4 treatment eliminated their ability to identify the glucose and fructose solutions. Experiment 2 asked whether 2 d of exposure to a 1 M glucose, fructose, or sucrose solution improved the identification of the same sugar solution. Following exposure, the B6 mice identified all 3 sugar solutions based on odor cues. Experiment 3 asked whether T1R3 knockout mice (i.e. mice lacking the T1R3 subunit of the T1R2 + R3 sweet taste receptor) could learn to discriminate 0.44 M glucose and fructose solutions based on odor cues. All mice were subjected to a 1-h preference test, both before and after exposure to the 0.44 M glucose and fructose solutions. During exposure, the experimental mice received ZnSO4 treatment, whereas the control mice received saline treatment. Before exposure, neither type of mouse preferred the glucose solution. After exposure, the control mice preferred the glucose solution, whereas the experimental mice did not. Our results reveal that mice can learn to use odor cues to identify and discriminate between sugar solutions.

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小鼠学会根据气味线索识别和区分糖溶液。
本研究探讨了嗅觉如何影响小鼠对糖溶液的摄食反应。实验 1 询问天真的 C57BL/6 (B6) 小鼠能否在 30 分钟的双瓶可接受性测试中根据气味线索识别 1M 葡萄糖、果糖或蔗糖溶液。我们在用 ZnSO4 处理小鼠使其丧失嗅觉之前和之后对其进行了测试。我们使用了两种间接测量气味介导反应的方法:开始试验的次数和开始舔食的潜伏期。在硫酸锌处理之前,小鼠学会了如何根据气味线索识别 1M 葡萄糖和果糖溶液(而不是蔗糖溶液)。硫酸锌处理消除了它们识别葡萄糖和果糖溶液的能力。实验 2 的问题是,接触 1M 葡萄糖、果糖或蔗糖溶液两天是否会提高对相同糖溶液的识别能力。接触后,B6 小鼠根据气味线索识别出了所有三种糖溶液。实验 3 询问 T1R3 基因敲除小鼠(即缺乏 T1R2+R3 甜味受体的 T1R3 亚基的小鼠)能否学会根据气味线索辨别 0.44M 葡萄糖和果糖溶液。所有小鼠在接触 0.44M 葡萄糖和果糖溶液之前和之后都接受了 1 小时的偏好测试。在接触过程中,实验组小鼠接受 ZnSO4 处理,而对照组小鼠接受生理盐水处理。接触前,两种小鼠都不喜欢葡萄糖溶液。接触后,对照组小鼠更喜欢葡萄糖溶液,而实验组小鼠则不喜欢。我们的研究结果表明,小鼠可以学会利用气味线索来识别和区分糖溶液。
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来源期刊
Chemical Senses
Chemical Senses 医学-行为科学
CiteScore
8.60
自引率
2.90%
发文量
25
审稿时长
1 months
期刊介绍: Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.
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