AlbuMAX supplemented media induces the formation of transmission-competent P. falciparum gametocytes

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular and biochemical parasitology Pub Date : 2024-05-31 DOI:10.1016/j.molbiopara.2024.111634

Wouter Graumans , Alex van der Starre , Rianne Stoter , Geert-Jan van Gemert , Chiara Andolina , Jordache Ramjith , Taco Kooij , Teun Bousema , Nicholas Proellochs

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Abstract

Asexual blood stage culture of Plasmodium falciparum is routinely performed but reproducibly inducing commitment to and maturation of viable gametocytes remains difficult. Culture media can be supplemented with human serum substitutes to induce commitment but these generally only allow for long-term culture of asexual parasites and not transmission-competent gametocytes due to their different lipid composition. Recent insights demonstrated the important roles lipids play in sexual commitment; elaborating on this we exposed ring stage parasites (20–24 hours hpi) for one day to AlbuMAX supplemented media to trigger induction to gametocytogenesis. We observed a significant increase in gametocytes after AlbuMAX induction compared to serum. We also tested the transmission potential of AlbuMAX inducted gametocytes and found a significant higher oocyst intensity compared to serum. We conclude that AlbuMAX supplemented media induces commitment, allows a more stable and predictable production of transmittable gametocytes than serum alone.

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AlbuMAX 补充培养基可诱导恶性疟原虫配子细胞的形成。

恶性疟原虫的无性血液阶段培养是常规操作，但要重复诱导有活力配子细胞的着床和成熟仍然很困难。可以在培养基中添加人血清替代物来诱导配子的形成，但由于人血清替代物的脂质成分不同，一般只能长期培养无性寄生虫，而不能培养具有传播能力的配子细胞。最近的研究表明，脂质在性承诺中发挥着重要作用；为了详细说明这一点，我们将环阶段寄生虫（20-24 小时 hpi）暴露在添加了 AlbuMAX 的培养基中一天，以诱导配子细胞的产生。与血清相比，我们观察到 AlbuMAX 诱导后配子细胞明显增加。我们还检测了AlbuMAX诱导配子细胞的传播潜力，发现与血清相比，卵囊强度明显更高。我们得出的结论是，与单纯的血清相比，添加了AlbuMAX的培养基能诱导出更稳定、更可预测的可传播配子细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.