Actin depolymerizing factor destrin governs cell migration in neural development during Xenopus embryogenesis

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecules and Cells Pub Date : 2024-06-01 DOI:10.1016/j.mocell.2024.100076

Youni Kim , Hyun-Kyung Lee , Kyeong-Yeon Park , Tayaba Ismail , Hongchan Lee , Hong-Yeoul Ryu , Dong-Hyung Cho , Taeg Kyu Kwon , Tae Joo Park , Taejoon Kwon , Hyun-Shik Lee

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Abstract

The actin-based cytoskeleton is considered a fundamental driving force for cell differentiation and development. Destrin (Dstn), a member of the actin-depolymerizing factor family, regulates actin dynamics by treadmilling actin filaments and increasing globular actin pools. However, the specific developmental roles of dstn have yet to be fully elucidated. Here, we investigated the physiological functions of dstn during early embryonic development using Xenopus laevis as an experimental model organism. dstn is expressed in anterior neural tissue and neural plate during Xenopus embryogenesis. Depleting dstn promoted morphants with short body axes and small heads. Moreover, dstn inhibition extended the neural plate region, impairing cell migration and distribution during neurulation. In addition to the neural plate, dstn knockdown perturbed neural crest cell migration. Our data suggest new insights for understanding the roles of actin dynamics in embryonic neural development, simultaneously presenting a new challenge for studying the complex networks governing cell migration involving actin dynamics.

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肌动蛋白解聚因子destrin在爪蟾胚胎发育过程中控制神经发育过程中的细胞迁移。

基于肌动蛋白的细胞骨架被认为是细胞分化和发育的基本动力。Destrin（dstn）是肌动蛋白解聚因子家族的一员，它通过踩踏肌动蛋白丝和增加球状肌动蛋白池来调节肌动蛋白的动态。然而，dstn 在发育过程中的具体作用尚未完全阐明。在此，我们以爪蟾为实验模型生物，研究了dstn在早期胚胎发育过程中的生理功能。dstn在爪蟾胚胎发育过程中表达于前部神经组织和神经板。抑制dstn可促进体轴短、头小的形态发生。此外，抑制 dstn 会扩大神经板区域，影响神经形成过程中的细胞迁移和分布。除神经板外，dstn敲除还扰乱了神经嵴细胞的迁移。我们的数据为理解肌动蛋白动力学在胚胎神经发育中的作用提供了新的见解，同时也为研究涉及肌动蛋白动力学的细胞迁移复杂网络提出了新的挑战。

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.

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