Encapsulation of Alpinia leaf essential oil in nanophytosome-embedded gel as novel strategy to treat periodontal infections: evaluation of antimicrobial effectiveness, pharmacokinetic, in vitro-ex vivo correlation and in silico studies.

IF 3 4区医学 Q2 CHEMISTRY, APPLIED Journal of microencapsulation Pub Date : 2024-08-01 Epub Date: 2024-06-03 DOI:10.1080/02652048.2024.2354234

Bhabani Sankar Satapathy, Abhishek Mishra, Sangram Keshari Biswal, Snigdha Pattnaik, Reena Parida, Biswabhusan Biswal, Tapan Kumar Shaw

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Abstract

Aim: The work reports a novel nanophytosomal gel encapsulating Alpinia galanga (L.) Willd leaf essential oil to treat periodontal infections.

Methods: Alpinia oil-loaded nanophytosomes (ANPs) were formulated by lipid layer hydration technique and were evaluated by FESEM, cryo-TEM, loading efficiency, zeta potential, particle size, release profile etc. Selected ANPs-loaded gel (ANPsG) was evaluated by both in vitro and in vivo methods.

Results: Selected ANPs were spherical, unilamellar, 49.32 ± 2.1 nm size, 0.45 PDI, -46.7 ± 0.8 mV zeta potential, 9.8 ± 0.5% (w/w) loading, 86.4 ± 3.02% (w/w) loading efficiency with sustained release profile. ANPsG showed good spreadability (6.8 ± 0.3 gm.cm/sec), extrudability (79.33 ± 1.5%), viscosity (36522 ± 0.82 cps), mucoadhesive strength (44.56 ± 3.5 gf) with sustained ex vivo release tendency. Satisfied ZOI and MIC was observed for ANPsG against periodontal bacteria vs. standard/control. ANPsG efficiently treated infection in ligature induced periodontitis model. Key pharmacokinetic parameters like AUC, MRT, V_d were enhanced for ANPsG.

Conclusion: ANPsG may be investigated for futuristic clinical studies.

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将阿尔卑斯叶精油封装在纳米叶绿体嵌入凝胶中作为治疗牙周感染的新策略：抗菌效果评估、药代动力学、体内外相关性和硅学研究。

目的：该研究报告了一种新型纳米叶绿体凝胶，其中封装了Alpinia galanga (L.) Willd叶精油，用于治疗牙周感染：方法：采用脂质层水合技术配制了Alpinia精油负载纳米叶绿体（ANPs），并通过FESEM、cryo-TEM、负载效率、zeta电位、粒度、释放曲线等进行了评估。通过体外和体内方法对选定的 ANPs 负载凝胶（ANPsG）进行了评估：结果：所选 ANPs 为球形、单拉美柱状，大小为 49.32 ± 2.1 nm，PDI 为 0.45，ZETA 电位为 -46.7 ± 0.8 mV，载量为 9.8 ± 0.5%（w/w），载量效率为 86.4 ± 3.02%（w/w），具有持续释放特性。ANPsG 显示出良好的铺展性（6.8 ± 0.3 gm.cm/sec）、挤出性（79.33 ± 1.5%）、粘度（36522 ± 0.82 cps）和粘附力（44.56 ± 3.5 gf），并具有体内持续释放趋势。与标准品/对照品相比，ANPsG 对牙周细菌的 ZOI 和 MIC 均令人满意。ANPsG 能有效治疗结扎诱导的牙周炎模型中的感染。ANPsG的AUC、MRT、Vd等关键药代动力学参数均有所提高：结论：ANPsG可用于未来的临床研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.