{"title":"Calocybe indica extract inhibits the growth and enzyme production of microbes that impair the healing of burn wounds","authors":"Sangeetha Chellapandi, Lalitha Vaidyanathan, Madhan Kumar Srinivasan, Juwala Malinika","doi":"10.21161/mjm.220099","DOIUrl":null,"url":null,"abstract":"Aims: The need for antimicrobial agents that could prevent the invasive spread of microbes that infect burn wounds directs the aim of the study. The edible mushroom Calocybe indica , with well-known biological activities, is explored to understand the role of the three bacteria, Streptococcus pyogenes , Enterococcus faecalis and Stenotrophomonas maltophilia, which are prevalent among the burn wounds, known for their tissue invasive properties and multidrug resistance. Methodology and results: The aqueous extract of C. indica was screened for antibacterial activity against the test microbes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the antibacterial aqueous extract were determined. The effect of the extract on the specific activity of the tissue-degrading enzymes protease and hyaluronidase was studied. The MIC of C. indica aqueous extract against the test organisms was found to be 125, 62.5 and 62.5 µg for S. pyogenes , S. maltophilia and E. faecalis , respectively. The specific activity of protease was reduced by 6, 1.3 and 3 folds, and hyaluronidase was reduced by 4.5, 2.8 and 4.1, respectively, in the order of test microbes mentioned above. Conclusion, significance and impact of study: The C. indica aqueous extracts with the potential to inhibit growth and retard invasive enzyme production in the microbes infecting burn wounds could be a significant therapeutic strategy in the management of clinical burn wounds to prevent the progression of the tissue damage. The bioactive component of the extract could further be identified analytically and formulated efficiently to enhance therapeutic applications.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Malaysian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21161/mjm.220099","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Aims: The need for antimicrobial agents that could prevent the invasive spread of microbes that infect burn wounds directs the aim of the study. The edible mushroom Calocybe indica , with well-known biological activities, is explored to understand the role of the three bacteria, Streptococcus pyogenes , Enterococcus faecalis and Stenotrophomonas maltophilia, which are prevalent among the burn wounds, known for their tissue invasive properties and multidrug resistance. Methodology and results: The aqueous extract of C. indica was screened for antibacterial activity against the test microbes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the antibacterial aqueous extract were determined. The effect of the extract on the specific activity of the tissue-degrading enzymes protease and hyaluronidase was studied. The MIC of C. indica aqueous extract against the test organisms was found to be 125, 62.5 and 62.5 µg for S. pyogenes , S. maltophilia and E. faecalis , respectively. The specific activity of protease was reduced by 6, 1.3 and 3 folds, and hyaluronidase was reduced by 4.5, 2.8 and 4.1, respectively, in the order of test microbes mentioned above. Conclusion, significance and impact of study: The C. indica aqueous extracts with the potential to inhibit growth and retard invasive enzyme production in the microbes infecting burn wounds could be a significant therapeutic strategy in the management of clinical burn wounds to prevent the progression of the tissue damage. The bioactive component of the extract could further be identified analytically and formulated efficiently to enhance therapeutic applications.
期刊介绍:
The Malaysian Journal of Microbiology (MJM) publishes high quality microbiology research related to the tropics. These include infectious diseases and antimicrobials. In addition, the journal also publishes research works on the application of microbes for the betterment of human society and the environment. The journal welcomes papers on isolation, identification, characterization and application of microbes and microbial products. The MJM is published under the auspices of the Malaysian Society for Microbiology. It serves as a forum for scientific communication among scientists and academics who deal with microbes and microbial products. The journal publishes research articles, short communications and review articles on various novel aspects of microbiology, which include topics related to medical, pharmaceutical, food, agricultural, industry, plant pathology, biotechnology, microbial genetics, environment, soil, water and biodeterioration. The journal aspires to emphasize the important roles played by microbes in our daily life.