Calocybe indica extract inhibits the growth and enzyme production of microbes that impair the healing of burn wounds

IF 0.4 Q4 MICROBIOLOGY Malaysian Journal of Microbiology Pub Date : 2024-06-01 DOI:10.21161/mjm.220099
Sangeetha Chellapandi, Lalitha Vaidyanathan, Madhan Kumar Srinivasan, Juwala Malinika
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Abstract

Aims: The need for antimicrobial agents that could prevent the invasive spread of microbes that infect burn wounds directs the aim of the study. The edible mushroom Calocybe indica , with well-known biological activities, is explored to understand the role of the three bacteria, Streptococcus pyogenes , Enterococcus faecalis and Stenotrophomonas maltophilia, which are prevalent among the burn wounds, known for their tissue invasive properties and multidrug resistance. Methodology and results: The aqueous extract of C. indica was screened for antibacterial activity against the test microbes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the antibacterial aqueous extract were determined. The effect of the extract on the specific activity of the tissue-degrading enzymes protease and hyaluronidase was studied. The MIC of C. indica aqueous extract against the test organisms was found to be 125, 62.5 and 62.5 µg for S. pyogenes , S. maltophilia and E. faecalis , respectively. The specific activity of protease was reduced by 6, 1.3 and 3 folds, and hyaluronidase was reduced by 4.5, 2.8 and 4.1, respectively, in the order of test microbes mentioned above. Conclusion, significance and impact of study: The C. indica aqueous extracts with the potential to inhibit growth and retard invasive enzyme production in the microbes infecting burn wounds could be a significant therapeutic strategy in the management of clinical burn wounds to prevent the progression of the tissue damage. The bioactive component of the extract could further be identified analytically and formulated efficiently to enhance therapeutic applications.
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菖蒲提取物可抑制影响烧伤伤口愈合的微生物的生长和酶的生成
研究目的:本研究的目的在于寻找抗菌剂,以防止烧伤伤口感染微生物的入侵传播。研究人员探索了具有著名生物活性的食用菌 Calocybe indica,以了解化脓性链球菌、粪肠球菌和嗜麦芽霉菌这三种细菌在烧伤伤口中的作用。方法和结果:筛选了 C. indica 的水提取物对测试微生物的抗菌活性。测定了抗菌水提取物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。研究了萃取物对组织降解酶蛋白酶和透明质酸酶特异活性的影响。发现 C. indica 水提取物对化脓性链球菌、嗜麦芽糖酸链球菌和粪大肠杆菌的 MIC 分别为 125、62.5 和 62.5 µg。按照上述试验微生物的顺序,蛋白酶的特异性活性分别降低了 6 倍、1.3 倍和 3 倍,透明质酸酶的特异性活性分别降低了 4.5 倍、2.8 倍和 4.1 倍。研究的结论、意义和影响:C.indica水提取物具有抑制烧伤创面感染微生物生长和延缓侵袭酶生成的潜力,可作为临床烧伤创面管理的重要治疗策略,防止组织损伤的恶化。提取物中的生物活性成分可以进一步分析鉴定,并进行有效配制,以加强治疗应用。
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来源期刊
CiteScore
0.70
自引率
0.00%
发文量
43
审稿时长
11 weeks
期刊介绍: The Malaysian Journal of Microbiology (MJM) publishes high quality microbiology research related to the tropics. These include infectious diseases and antimicrobials. In addition, the journal also publishes research works on the application of microbes for the betterment of human society and the environment. The journal welcomes papers on isolation, identification, characterization and application of microbes and microbial products. The MJM is published under the auspices of the Malaysian Society for Microbiology. It serves as a forum for scientific communication among scientists and academics who deal with microbes and microbial products. The journal publishes research articles, short communications and review articles on various novel aspects of microbiology, which include topics related to medical, pharmaceutical, food, agricultural, industry, plant pathology, biotechnology, microbial genetics, environment, soil, water and biodeterioration. The journal aspires to emphasize the important roles played by microbes in our daily life.
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