Fifty years after the first identification of Toscana virus in Italy: Genomic characterization of viral isolates within lineage A and aminoacidic markers of evolution

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES Infection Genetics and Evolution Pub Date : 2024-06-01 DOI:10.1016/j.meegid.2024.105601

Giulia Marsili , Carlo Pallotto , Claudia Fortuna , Antonello Amendola , Cristiano Fiorentini , Sara Esperti , Pierluigi Blanc , Lorenzo Roberto Suardi , Venturi Giulietta , Claudio Argentini

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Abstract

Toscana Virus (TosV) was firstly isolated from phlebotomine in our Institute about fifty years ago. Later, in 1984–1985, TosV infection, although asymptomatic in most cases, was shown to cause disease in humans, mainly fever and meningitis. By means of genetic analysis of part of M segment, we describe 3 new viral isolates obtained directly from cerebrospinal fluid or sera samples of patients diagnosed with TosV infection in July 2020 in Tuscany region. Phylogenesis was used to propose the clustering of TosV lineage A strains in 3 main groups, whereas deep mutational analysis based on 12 amino acid positions, allowed the identification of 9 putative strains. We discuss deep mutational analysis as a method to identify molecular signature of host adaptation and/or pathogenesis.

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意大利首次发现托斯卡纳病毒五十年后：A系病毒分离株的基因组特征和进化的氨基酸标记。

托斯卡纳病毒（TosV）大约五十年前首次从我们研究所的血吸虫中分离出来。后来，在 1984-1985 年间，TosV 感染虽然在大多数情况下无症状，但被证明可导致人类疾病，主要是发烧和脑膜炎。通过对 M 段的部分基因进行分析，我们描述了 3 种新的病毒分离物，它们直接来自 2020 年 7 月在托斯卡纳地区被诊断为 TosV 感染患者的脑脊液或血清样本。我们利用系统发育法将 TosV A 系毒株分为 3 个主要群组，而基于 12 个氨基酸位点的深度突变分析则确定了 9 个假定毒株。我们将深度突变分析作为确定宿主适应和/或致病分子特征的一种方法进行了讨论。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .