Micronucleus test in reptiles: Current and future perspectives

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-05-23 DOI:10.1016/j.mrgentox.2024.503772

L.M. Odetti , E.V. Paravani , Ma.F. Simoniello , G.L. Poletta

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Abstract

Micronucleus (MN) cell counting emerged in 1973–1975 as a valid alternative for characterizing chromosomal damage caused by different agents. It was first described in mammals, but its application was rapidly extended to other vertebrates, mainly fish. However, it was not until 28 years later that this test was implemented in studies on reptiles. Nowadays, reptiles are found to be excellent non-target species from environmental contamination exposure and MN test has become a fundamental tool for analyzing genotoxic effects induced by various xenobiotics. In this article we provide an updated review of the application of the MN test in reptile species, from an ecotoxicological perspective. Therefore, we present (I) a bibliometric analysis of the available research on genotoxic-induced MN formation in reptile species; (II) the use of reptiles as sentinel organisms in ecotoxicological studies; and (III) the strength and weakness of the application of the MN test in this group. With this review, we aim to provide a comprehensive view on the use of the MN test in ecotoxicology and to encourage further studies involving reptile species.

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爬行动物的微核试验：当前和未来展望

微核（MN）细胞计数兴起于 1973-1975 年，是描述不同媒介造成的染色体损伤的有效替代方法。该方法最初是在哺乳动物中应用，但其应用范围迅速扩展到其他脊椎动物，主要是鱼类。然而，直到 28 年后，爬行动物研究中才开始使用这种检测方法。如今，人们发现爬行动物是环境污染暴露的极佳非目标物种，MN 试验已成为分析各种异种生物诱导的遗传毒性效应的基本工具。在本文中，我们从生态毒理学的角度，对 MN 试验在爬行动物物种中的应用进行了最新综述。因此，我们将介绍：(I) 对爬行动物物种中基因毒性诱导的 MN 形成的现有研究进行文献计量分析；(II) 在生态毒理学研究中使用爬行动物作为哨兵生物；(III) MN 试验在这一群体中应用的优势和劣势。本综述旨在提供生态毒理学中使用 MN 试验的全面观点，并鼓励进一步开展涉及爬行动物物种的研究。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.