A fluorescent probe based on cyclochalcone for detecting peroxynitrite.

IF 2.7 3区 化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Photochemical & Photobiological Sciences Pub Date : 2024-06-01 Epub Date: 2024-06-05 DOI:10.1007/s43630-024-00565-y
Li-Hao Liu, Xian-Zhao Shang, Jian-Hao Yuan, Yi-Ning Luo, Jia-Yi Wang, Xiao-Lei Xue, Nan Jiang, Kun-Peng Wang, Zhi-Qiang Hu
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Abstract

A novel cyclic chalcone fluorescent probe C-PN was synthesized to detect ONOO-. After reaction with peroxynitrite, the double bond of C-PN in the cyclic chalcone structure was disconnected, which caused the change of intramolecular charge transfer (ICT) effect, emitting blue fluorescence and quenching orange red fluorescence. Visible to the naked eye, the color of the probe solution changed. The probe showed low sensitivity (detection limit = 20.2 nm), short response time (less than 60 s) at low concentration of ONOO-, good visibility, and good selectivity and stability for ONOO-.

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基于环查耳酮的荧光探针,用于检测过氧化亚硝酸盐。
研究人员合成了一种新型环查尔酮荧光探针C-PN,用于检测ONOO-。与过亚硝酸反应后,环状查尔酮结构中的 C-PN 双键断开,导致分子内电荷转移效应发生变化,发出蓝色荧光并淬灭橙红色荧光。肉眼可见,探针溶液的颜色发生了变化。该探针灵敏度低(检测限 = 20.2 nm),在低浓度 ONOO- 条件下响应时间短(小于 60 s),能见度高,对 ONOO- 具有良好的选择性和稳定性。
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来源期刊
Photochemical & Photobiological Sciences
Photochemical & Photobiological Sciences 生物-生化与分子生物学
CiteScore
5.60
自引率
6.50%
发文量
201
审稿时长
2.3 months
期刊介绍: A society-owned journal publishing high quality research on all aspects of photochemistry and photobiology.
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