Polyoxometalate functionalized magnetic metal-organic framework with multi-affinity sites for efficient enrichment of phosphopeptides.

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2024-08-01 Epub Date: 2024-06-05 DOI:10.1007/s00216-024-05365-y

Dandan Jiang, Ruixue Qi, Siyu Wu, Yangyang Li, Jinghai Liu

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Abstract

The reasonable design of metal-organic framework (MOF)-derived nanomaterial has important meaning in increasing the enrichment efficiency in the study of protein phosphorylation. In this work, a polyoxometalate (POM) functionalized magnetic MOF nanomaterial (Fe₃O₄@MIL-125-POM) was designed and fabricated. The nanomaterial with multi-affinity sites (unsaturated metal sites and metal oxide clusters) was used for the enrichment of phosphopeptides. Fe₃O₄@MIL-125-POM had high-efficient enrichment performance towards phosphopeptides (selectivity, a mass ratio of bovine serum albumin/α-casein/β-casein at 5000:1:1; sensitivity, 0.1 fmol; satisfactory repeatability, ten times). Furthermore, Fe₃O₄@MIL-125-POM was employed to enrich phosphopeptides from non-fat milk digests, saliva, serum, and A549 cell lysate. The enrichment results illustrated the great potential of Fe₃O₄@MIL-125-POM for efficient identification of low-abundance phosphopeptides.

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具有多亲和位点的聚氧化金属功能化磁性金属有机框架，用于高效富集磷酸肽。

合理设计由金属有机框架（MOF）衍生的纳米材料对于提高蛋白质磷酸化研究的富集效率具有重要意义。本研究设计并制备了一种聚氧化金属（POM）功能化磁性 MOF 纳米材料（Fe3O4@MIL-125-POM）。该纳米材料具有多亲和位点（不饱和金属位点和金属氧化物簇），用于富集磷酸肽。Fe3O4@MIL-125-POM对磷酸肽具有高效的富集性能（选择性，牛血清白蛋白/α-酪蛋白/β-酪蛋白的质量比为5000:1:1；灵敏度，0.1 fmol；重复性，10次）。此外，Fe3O4@MIL-125-POM 还被用于富集非脂奶消化物、唾液、血清和 A549 细胞裂解物中的磷酸肽。富集结果表明，Fe3O4@MIL-125-POM 在高效鉴定低丰度磷酸肽方面具有巨大潜力。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.