Galectin-3 inhibition alleviated LPS-induced periodontal inflammation in gingival fibroblasts and experimental periodontitis mice.

IF 6.7 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL Clinical science Pub Date : 2024-06-19 DOI:10.1042/CS20240036
Song Wenjing, Liu Mengmeng, Shang Lingling, Ding Tian, Kang Wenyan, Ge Shaohua
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Abstract

Objectives: Clinical studies have confirmed that galectin-3 (Gal-3) levels are significantly elevated in periodontitis patients. The present study aimed to explore the effects of Gal-3 inhibition on periodontal inflammation in vitro and in vivo.

Methods: Human gingival fibroblasts (HGFs) with or without Gal-3 knockdown were stimulated by lipopolysaccharide (LPS), and a ligation-induced mouse periodontitis model treated with a Gal-3 inhibitor was established. Hematoxylin-eosin (H&E) and immunohistochemistry (IHC) staining were used to evaluate Gal-3 levels in gingival tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect Gal-3, interleukin (IL)-6, IL-8, and C-C motif ligand 2 (CCL2) expression. Immunofluorescence and western blotting were used to detect NF-κB and ERK signaling pathway activation. Micro-computed tomography was used to analyse the degree of bone loss.

Results: Gal-3 was significantly up-regulated in inflamed gingival tissues and LPS-induced HGFs. Gal-3 knockdown markedly decreased LPS-induced IL-6, IL-8, and CCL2 expression and blocked NF-κB and ERK signaling pathway activation in HGFs. In the mouse periodontitis model, Gal-3 inhibition significantly alleviated IL-1β and IL-6 infiltration in gingival tissue and mitigated periodontal bone loss.

Conclusions: Gal-3 inhibition notably alleviated periodontal inflammation partly through blocking NF-κB and ERK signaling pathway activation.

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抑制 Galectin-3 可减轻 LPS 诱导的牙龈成纤维细胞和实验性牙周炎小鼠的牙周炎症。
目的:临床研究证实,牙周炎患者体内的galectin-3(Gal-3)水平显著升高。方法:用脂多糖(LPS)刺激敲除或未敲除 Gal-3 的人牙龈成纤维细胞(HGFs),并用 Gal-3 抑制剂建立结扎诱导的小鼠牙周炎模型。采用血红素-伊红(H&E)和免疫组化(IHC)染色法评估牙龈组织中的 Gal-3 水平。实时定量聚合酶链反应(ԛRT-PCR)和酶联免疫吸附试验(ELISA)用于检测 Gal-3、白细胞介素(IL)-6、IL-8 和 C-C motif ligand 2(CCL2)的表达。免疫荧光和 Western 印迹技术用于检测 NF-ᵰ5;B 和 ERK 信号通路的激活情况。显微计算机断层扫描用于分析骨质流失的程度:结果:在发炎的牙龈组织和 LPS 诱导的 HGFs 中,Gal-3 明显上调。结果:Gal-3在发炎的牙龈组织和LPS诱导的HGFs中明显上调,Gal-3敲除可明显降低LPS诱导的IL-6、IL-8和CCL2的表达,并阻断HGFs中NF-ᵰ5;B和ERK信号通路的激活。在小鼠牙周炎模型中,Gal-3抑制剂能显著缓解牙龈组织中IL-1β和IL-6的浸润,减轻牙周骨质流失:结论:Gal-3抑制剂通过阻断NF-ᵰ5;B和ERK信号通路的激活,明显减轻了牙周炎症。
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来源期刊
Clinical science
Clinical science 医学-医学:研究与实验
CiteScore
11.40
自引率
0.00%
发文量
189
审稿时长
4-8 weeks
期刊介绍: Translating molecular bioscience and experimental research into medical insights, Clinical Science offers multi-disciplinary coverage and clinical perspectives to advance human health. Its international Editorial Board is charged with selecting peer-reviewed original papers of the highest scientific merit covering the broad spectrum of biomedical specialities including, although not exclusively: Cardiovascular system Cerebrovascular system Gastrointestinal tract and liver Genomic medicine Infection and immunity Inflammation Oncology Metabolism Endocrinology and nutrition Nephrology Circulation Respiratory system Vascular biology Molecular pathology.
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