Comparison of microRNA expression levels in patients with schizophrenia before and after electroconvulsive therapy.

IF 1.5 4区医学 Q4 GENETICS & HEREDITY Psychiatric Genetics Pub Date : 2024-08-01 Epub Date: 2024-05-29 DOI:10.1097/YPG.0000000000000371

Nazife Gamze Usta Saglam, Mehmet Bugrahan Duz, Seda Salman Yilmaz, Mustafa Ozen, Ibrahim Balcioglu

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Abstract

Objective: Exploring the role of microRNAs in the antipsychotic efficacy of electroconvulsive therapy (ECT) will contribute to understanding the underlying mechanism through which ECT exerts its therapeutic effects. The primary objective of this study was to identify microRNA alterations before and after ECT in patients with schizophrenia.

Methods: We compared microarray-based microRNA profiles in peripheral blood from eight patients with schizophrenia before and after ECT and eight healthy controls. Then, we aimed to validate selected differentially expressed microRNAs in 30 patients with schizophrenia following a course of ECT, alongside 30 healthy controls by using quantitative reverse-transcription PCR.

Results: Microarray-based expression profiling revealed alterations in 681 microRNAs when comparing pre- and post-ECT samples. Subsequent quantitative reverse-transcription PCR analysis of the selected microRNAs (miR-20a-5p and miR-598) did not reveal any statistical differences between pre- and post-ECT samples nor between pre-ECT samples and those of healthy controls.

Conclusion: As neuroepigenetic studies on ECT are still in their infancy, the results reported in this study are best interpreted as exploratory outcomes. Additional studies are required to explore the potential epigenetic mechanisms underlying the therapeutic efficacy of ECT.

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电休克治疗前后精神分裂症患者体内微RNA表达水平的比较。

探索微RNA在电休克疗法（ECT）抗精神病疗效中的作用将有助于了解ECT发挥治疗效果的内在机制。本研究的主要目的是确定精神分裂症患者在电休克疗法前后的微RNA变化。我们比较了 ECT 前后 8 名精神分裂症患者和 8 名健康对照者外周血中基于芯片的 microRNA 图谱。然后，我们利用定量实时 PCR（qRT-PCR）技术，对 30 名接受过 ECT 治疗的精神分裂症患者和 30 名健康对照者的选定差异表达 microRNA 进行了验证。基于芯片的表达谱分析显示，在比较ECT前后的样本时，有681个microRNA发生了变化。随后对选定的微RNA（miR-20a-5p和miR-598）进行的qRT-PCR分析未发现ECT前后样本之间或ECT前样本与健康对照组样本之间存在任何统计学差异。由于有关 ECT 的神经表观遗传学研究仍处于起步阶段，本研究报告的结果最好被解释为探索性结果。还需要进行更多的研究，以探索 ECT 疗效背后潜在的表观遗传学机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Psychiatric Genetics 医学-神经科学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： The journal aims to publish papers which bring together clinical observations, psychological and behavioural abnormalities and genetic data. All papers are fully refereed. Psychiatric Genetics is also a forum for reporting new approaches to genetic research in psychiatry and neurology utilizing novel techniques or methodologies. Psychiatric Genetics publishes original Research Reports dealing with inherited factors involved in psychiatric and neurological disorders. This encompasses gene localization and chromosome markers, changes in neuronal gene expression related to psychiatric disease, linkage genetics analyses, family, twin and adoption studies, and genetically based animal models of neuropsychiatric disease. The journal covers areas such as molecular neurobiology and molecular genetics relevant to mental illness. Reviews of the literature and Commentaries in areas of current interest will be considered for publication. Reviews and Commentaries in areas outside psychiatric genetics, but of interest and importance to Psychiatric Genetics, will also be considered. Psychiatric Genetics also publishes Book Reviews, Brief Reports and Conference Reports.