Chromatographic and Spectrophotometric Determination of Clindamycin in Pharmaceutical Products

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-06-01 DOI:10.22146/ijc.91599

I. Al-Momani, Lana Mohammad Zaid Al-Kilani

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Abstract

Accurate, precise, and reliable chromatographic and spectrophotometric methods were developed for determining clindamycin (CLD) in pharmaceutical formulations. The spectrophotometric method was adopted for flow injection analysis (FIA). The method is based on the online oxidation of CLD and measuring the absorbance of the resulting product using a flow cell at 605 nm. Experimental conditions, including FIA variables and reaction conditions, were optimized. The chromatographic separation was achieved using a C8 column and an isocratic mobile phase. The composition of the mobile phase selected for the analysis consists of a mixture of phosphate buffer (50%), MeOH (35%), and ACN (15%), adjusted to a pH of 3.47 by phosphoric acid. The eluent was monitored with a UV detector at 205 nm. The linearity range was 10–200 and 50–800 µg/mL for the FIA and HPLC, respectively. The applicability of the FIA and HPLC methods was validated by analyzing CLD in synthetic and commercial pharmaceutical products. No significant interferences were observed from the common excipients usually used in commercial formulations.

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色谱法和分光光度法测定药品中的克林霉素

建立了准确、精确、可靠的色谱法和分光光度法测定药物制剂中的克林霉素（CLD）。采用分光光度法进行流动注射分析（FIA）。该方法基于 CLD 的在线氧化，并使用流动池在 605 纳米波长处测量生成物的吸光度。对包括 FIA 变量和反应条件在内的实验条件进行了优化。色谱分离采用 C8 色谱柱和等度流动相。用于分析的流动相由磷酸盐缓冲液（50%）、MeOH（35%）和 ACN（15%）的混合物组成，用磷酸调节 pH 值至 3.47。洗脱液用紫外检测器在 205 纳米波长处进行监测。FIA 和 HPLC 的线性范围分别为 10-200 微克/毫升和 50-800 微克/毫升。通过分析合成和商业药品中的 CLD，验证了 FIA 和 HPLC 方法的适用性。没有发现商业制剂中常用辅料的明显干扰。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.