Low-energy red light-emitting diode irradiation enhances osteogenic differentiation of periodontal ligament stem cells by regulating miR-146a-5p

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontal research Pub Date : 2024-06-06 DOI:10.1111/jre.13276

Yajiao Ren, Shifen Wang, Hao Li, Jiaxin Li, Xiaorong Lan, Yao Wang

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Abstract

Aims

The study aimed to investigate the role of miR-146a-5p in osteogenesis of hPDLSCs irradiated with low-energy red LEDs.

Methods

After irradiation with 5 J/cm² red LED, miR-146a-5p expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR), and osteogenic markers expression was determined by RT-qPCR and Western blotting. Alkaline phosphatase (ALP) activity was assessed by ALP staining, and mineralization was assessed by Alizarin Red staining, respectively. Lentiviral vectors were designed to regulate miR-146a-5p expression. Dual-luciferase reporter assay was performed to confirm the targeted relationship between miR-146a-5p and MAPK1. Short hairpin RNA (shRNA) was used to regulate MAPK1 expression.

Results

RT-qPCR and western blotting revealed that 5 J/cm² irradiation elevated the levels of the osteogenic markers osterix (OSX) and bone sialoprotein (BSP) in hPDLSCs. miR-146a-5p is downregulated in hPDLSCs under the low-energy red LED light irradiation. miR-146a-5p underexpression markedly promoted the osteogenic potential of hPDLSCs. miR-146a-5p targeted MAPK1. 5 J/cm² red LED irradiation rescued the inhibitory effects of upregulated miR-146a-5p on osteogenic differentiation, and the positive influence of red LED irradiation could be reversed by downregulated MAPK1.

Conclusion

These findings confirm that miR-146a-5p is involved in the effect of LED irradiation on the osteogenic differentiation of hPDLSCs by targeting MAPK1. Red LED irradiation may be a potential clinical adjunct therapy for periodontal regeneration.

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低能量红色发光二极管照射通过调节 miR-146a-5p 增强牙周韧带干细胞的成骨分化。

目的：本研究旨在探讨miR-146a-5p在低能量红光LED照射hPDLSCs成骨过程中的作用：方法：用 5 J/cm2 红色 LED 照射 hPDLSCs 后，通过实时定量聚合酶链反应（RT-qPCR）检测 miR-146a-5p 的表达，并通过 RT-qPCR 和 Western 印迹检测成骨标志物的表达。碱性磷酸酶（ALP）活性通过ALP染色评估，矿化度通过茜素红染色评估。设计了慢病毒载体来调控 miR-146a-5p 的表达。双荧光素酶报告实验证实了 miR-146a-5p 与 MAPK1 之间的靶向关系。用短发夹RNA（shRNA）调控MAPK1的表达：miR-146a-5p靶向MAPK1。5焦耳/平方厘米的红色LED照射可挽救上调的miR-146a-5p对成骨分化的抑制作用，红色LED照射的积极影响可被下调的MAPK1逆转：这些研究结果证实，miR-146a-5p通过靶向MAPK1参与了LED照射对hPDLSCs成骨分化的影响。红光LED照射可能是一种潜在的牙周再生临床辅助疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.