In vitro Assessment of Free Radical Scavenging Effect and Thermal Protein Denaturation Inhibition of Bee Venom for an Anti-Inflammatory Use.

Current health sciences journal Pub Date : 2024-01-01 Epub Date: 2024-03-31 DOI:10.12865/CHSJ.50.01.11
Camelia Alina Gîlcescu Florescu, Elena Camelia Stanciulescu, Anca Berbecaru-Iovan, Roxana Maria Balasoiu, Catalina Gabriela Pisoschi
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Abstract

Inflammation and the injuries produced by free radicals are interconnected and influence each other. The underlying mechanisms of inflammation are partially attributed to the release of free radicals by immune cells, prooxidants that can also cause protein alteration. This study was performed in order to assess the potential anti-inflammatory effect of two bee venom samples harvested from Apis mellifera. Free radical scavenging capacity was investigated using DPPH and ABTS.+ tests and protective effect on proteins through the inhibitory activity on thermal denaturation of albumin.

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体外评估蜂毒的自由基清除效果和热蛋白变性抑制作用,以用于抗炎。
炎症和自由基造成的伤害是相互关联、相互影响的。炎症的根本机制部分归因于免疫细胞释放的自由基,这些原氧化剂也会导致蛋白质改变。本研究旨在评估从蜂毒中提取的两种蜂毒样本的潜在抗炎作用。使用 DPPH 和 ABTS.+ 试验研究了清除自由基的能力,并通过抑制白蛋白热变性的活性研究了对蛋白质的保护作用。
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