In vitro assessment of the genotoxic and cytotoxic effects of clarified açai (Euterpe oleracea MART) extract in a gastric cancer cell line (AGP01 cells).

IF 2.6 3区医学 Q3 TOXICOLOGY Toxicology in Vitro Pub Date : 2024-06-06 DOI:10.1016/j.tiv.2024.105873

Thiago S. Santos , Marcelo O. Bahia , Adriana C. Guimarães , Carolina R.T. Souza , Nilton A. Muto , Hervé Rogez , Rommel M.R. Burbano

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Abstract

Açaí (Euterpe oleracea MART) is a fruit of great importance for the Amazon region in nutritional, cultural and socioeconomic terms. In recent years, açaí has been the subject of several studies due to its beneficial properties for health, including effects against tumor cells. Therefore, the present work aimed to evaluate in vitro the genotoxic and cytotoxic effects of the clarified extract of açaí juice in a human metastatic gastric cancer cell line (AGP01 cells). For comparison purposes, a non-transformed cell line of African green monkey renal epithelial cells (VERO cells) was used. The viability assay by resazurin reduction, the comet assay, the determination of cell death by differential fluorescent dyes and the wound healing migration assay were performed. A reduction in viability was observed only in the AGP01 line within 72 h. There was no genotoxic damage or cell death (through apoptosis or necrosis) in any of the cell lines. However, açaí extract induced motility reduction in both cell lines. The reduction in cell viability and the induction of the anti-migratory effect in the AGP01 cell line opens perspectives for exploring the potential of açaí as an adjuvant in the treatment of gastric cancer.

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体外评估阿萨伊（Euterpe oleracea MART）提取物在胃癌细胞系（AGP01 细胞）中的基因毒性和细胞毒性作用。

阿萨伊（Euterpe oleracea MART）是亚马逊地区一种在营养、文化和社会经济方面都非常重要的水果。近年来，阿萨伊因其有益健康的特性，包括对肿瘤细胞的作用，已成为多项研究的主题。因此，本研究旨在体外评估阿萨伊果汁澄清提取物对人类转移性胃癌细胞系（AGP01 细胞）的遗传毒性和细胞毒性作用。为了进行比较，还使用了非洲绿猴肾上皮细胞（VERO 细胞）的非转化细胞系。进行了还原瑞香素活力测定、彗星测定、差异荧光染料细胞死亡测定和伤口愈合迁移测定。结果表明，只有 AGP01 株在 72 小时内出现了活力下降。所有细胞系都没有出现基因毒性损伤或细胞死亡（凋亡或坏死）。不过，阿萨伊提取物会导致两种细胞系的运动能力下降。AGP01细胞系中细胞活力的降低和抗迁移效应的诱导为探索阿萨伊作为治疗胃癌的辅助剂的潜力开辟了前景。

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来源期刊

Toxicology in Vitro 医学-毒理学

CiteScore

6.50

自引率

3.10%

发文量

181

审稿时长

65 days

期刊介绍： Toxicology in Vitro publishes original research papers and reviews on the application and use of in vitro systems for assessing or predicting the toxic effects of chemicals and elucidating their mechanisms of action. These in vitro techniques include utilizing cell or tissue cultures, isolated cells, tissue slices, subcellular fractions, transgenic cell cultures, and cells from transgenic organisms, as well as in silico modelling. The Journal will focus on investigations that involve the development and validation of new in vitro methods, e.g. for prediction of toxic effects based on traditional and in silico modelling; on the use of methods in high-throughput toxicology and pharmacology; elucidation of mechanisms of toxic action; the application of genomics, transcriptomics and proteomics in toxicology, as well as on comparative studies that characterise the relationship between in vitro and in vivo findings. The Journal strongly encourages the submission of manuscripts that focus on the development of in vitro methods, their practical applications and regulatory use (e.g. in the areas of food components cosmetics, pharmaceuticals, pesticides, and industrial chemicals). Toxicology in Vitro discourages papers that record reporting on toxicological effects from materials, such as plant extracts or herbal medicines, that have not been chemically characterized.