Comparative study on muscle function in two different streptozotocin-induced diabetic models

IF 3.1 3区医学 Q2 ENDOCRINOLOGY & METABOLISM Acta Diabetologica Pub Date : 2024-06-10 DOI:10.1007/s00592-024-02311-3

Rahmawati Aisyah, Mion Kamesawa, Mayu Horii, Daiki Watanabe, Yuki Yoshida, Kenshu Miyata, Thanutchaporn Kumrungsee, Masanobu Wada, Noriyuki Yanaka

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Abstract

Aims

Streptozotocin (STZ) is widely used to study diabetic complications. Owing to the nonspecific cytotoxicity of high-dose STZ, alternative models using moderate-dose or a combination of low-dose STZ and a high-fat diet have been established. This study aimed to investigate the effects of these models on muscle function.

Methods

The muscle function of two STZ models using moderate-dose STZ (100 mg/kg, twice) and a combination of low-dose STZ and high-fat diet (50 mg/kg for 5 consecutive days + 45% high-fat diet) were examined using in vivo electrical stimulation. Biochemical and gene expression analysis were conducted on the skeletal muscles of the models immediately after the stimulation.

Results

The contractile force did not differ significantly between the models compared to respective controls. However, the moderate-dose STZ model showed more severe fatigue and blunted exercise-induced glycogen degradation possibly thorough a downregulation of oxidative phosphorylation- and vasculature development-related genes expression.

Conclusions

Moderate-dose STZ model is suitable for fatigability assessment in diabetes and careful understanding on the molecular signatures of each model is necessary to guide the selection of suitable models to study diabetic myopathy.

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两种不同链脲佐菌素诱导糖尿病模型肌肉功能的比较研究

目的：链脲佐菌素（STZ）被广泛用于研究糖尿病并发症。由于高剂量 STZ 的非特异性细胞毒性，人们建立了使用中等剂量或低剂量 STZ 和高脂肪饮食组合的替代模型。本研究旨在探讨这些模型对肌肉功能的影响：方法：使用体内电刺激法检测了使用中等剂量 STZ（100 毫克/千克，两次）和低剂量 STZ 与高脂肪饮食（50 毫克/千克，连续 5 天 + 45% 高脂肪饮食）组合的两种 STZ 模型的肌肉功能。刺激后立即对模型的骨骼肌进行生化和基因表达分析：结果：与各自的对照组相比，模型的收缩力没有明显差异。然而，中度剂量的 STZ 模型表现出更严重的疲劳，运动诱导的糖原降解减弱，这可能是由于氧化磷酸化和血管发育相关基因表达下调所致：结论：中等剂量 STZ 模型适用于糖尿病患者的疲劳评估，有必要仔细了解每种模型的分子特征，以指导选择合适的模型来研究糖尿病肌病。

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来源期刊

Acta Diabetologica 医学-内分泌学与代谢

CiteScore

7.30

自引率

2.60%

发文量

180

审稿时长

2 months

期刊介绍： Acta Diabetologica is a journal that publishes reports of experimental and clinical research on diabetes mellitus and related metabolic diseases. Original contributions on biochemical, physiological, pathophysiological and clinical aspects of research on diabetes and metabolic diseases are welcome. Reports are published in the form of original articles, short communications and letters to the editor. Invited reviews and editorials are also published. A Methodology forum, which publishes contributions on methodological aspects of diabetes in vivo and in vitro, is also available. The Editor-in-chief will be pleased to consider articles describing new techniques (e.g., new transplantation methods, metabolic models), of innovative importance in the field of diabetes/metabolism. Finally, workshop reports are also welcome in Acta Diabetologica.