Group 3 innate lymphoid cells promotes Th17 cells differentiation in rheumatoid arthritis.

IF 3.4 4区 医学 Q2 RHEUMATOLOGY Clinical and experimental rheumatology Pub Date : 2024-11-01 Epub Date: 2024-06-06 DOI:10.55563/clinexprheumatol/ktbiqc
Xinhang Liu, Lisheng Wu, Shixian Chen, Xiao Liang, Xiaoyun Chen, Xiaoguang Chen, Juan Li, Junqing Zhu
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Abstract

Objectives: To investigate the correlation between innate lymphoid cell (ILC) subsets with T-helper (Th) cells and to explore the effect of ILCs on T cells in rheumatoid arthritis (RA).

Methods: We analysed the frequencies of ILC subsets in RA patients with varying disease activity and their correlation with Th cell subsets. We further investigated this correlation in various organs of collagen-induced arthritis (CIA) mice. The effects of ILCs on CD4+ T cells were determined by in vitro cell co-culture experiments.

Results: ILCs were less frequent in RA patients than in healthy controls, with higher levels of group 3 ILCs (ILC3s) in RA (p<0.05). ILC3s correlated positively with Th1 and Th17 cells in RA peripheral blood (p<0.05). In the peripheral blood, spleen, and lymph nodes of CIA, ILC3s decreased and then increased during arthritis progression. ILC3s correlated positively with Th1 and Th17 cells in the spleen and lymph nodes of CIA (p<0.05). NKp46+ ILC3s in the spleen positively correlated with Th1 and Th17 cells (p<0.05). Under Th17 cell differentiation conditions, co-culturing CIA-derived ILC3s directly with naive CD4+ T cells promoted Th17 differentiation and increased IL-17 secretion. However, co-culturing through a transwell insert impeded Th17 differentiation without affecting IL-17 secretion.

Conclusions: ILC3s positively correlated with Th1 and Th17 cells in RA. In CIA, the frequencies of ILC3s changed with disease development and showed a positive correlation with Th1 and Th17 cells. ILC3s may facilitate the differentiation of Th17 cells through direct cell-cell contact.

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第 3 组先天性淋巴细胞促进类风湿性关节炎中 Th17 细胞的分化。
目的研究先天性淋巴细胞(ILC)亚群与T辅助细胞(Th)之间的相关性,并探讨ILC对类风湿性关节炎(RA)中T细胞的影响:我们分析了不同疾病活动度的 RA 患者中 ILC 亚群的频率及其与 Th 细胞亚群的相关性。我们在胶原诱导关节炎(CIA)小鼠的不同器官中进一步研究了这种相关性。通过体外细胞共培养实验确定了 ILCs 对 CD4+ T 细胞的影响:结果:与健康对照组相比,ILCs在RA患者中的出现频率较低,而在RA患者中第3组ILCs(ILC3s)的水平较高(p结论:ILC3s与CD4+ T细胞呈正相关:ILC3s与RA中的Th1和Th17细胞呈正相关。在CIA中,ILC3s的频率随着疾病的发展而变化,并与Th1和Th17细胞呈正相关。ILC3s可能通过直接的细胞-细胞接触促进Th17细胞的分化。
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来源期刊
CiteScore
6.10
自引率
18.90%
发文量
377
审稿时长
3-6 weeks
期刊介绍: Clinical and Experimental Rheumatology is a bi-monthly international peer-reviewed journal which has been covering all clinical, experimental and translational aspects of musculoskeletal, arthritic and connective tissue diseases since 1983.
期刊最新文献
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