Low Prevalence of Anti-HBc Antibody and Lack of HBV DNA Among HBsAg-Negative Blood Donors in Iran: A Cross-sectional Study and Review of Literature.

IF 1 4区医学 Q3 MEDICINE, GENERAL & INTERNAL Archives of Iranian Medicine Pub Date : 2024-06-01 Epub Date: 2024-04-27 DOI:10.34172/aim.28579

Mohammad Reza Hedayati-Moghaddam, Farahnaz Tehranian, Arman Mosavat, Rahele Miri, Sanaz Ahmadi Ghezeldasht

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Abstract

Background: Occult hepatitis B infection (OBI) refers to the presence of hepatitis B virus (HBV) DNA in the serum or liver of individuals who tested negative for HBV surface antigen (HBsAg). This study aimed to determine seropositivity for antibodies against HBV core antigen (anti-HBc) and the frequency of OBI among the HBsAg non-reactive blood donors in Mashhad, northeastern Iran.

Methods: In this cross-sectional study, serum samples of HBsAg-negative blood donors were examined for anti-HBc during June and August 2018. Anti-HBc-positive samples were tested for antibodies against HBsAg (anti-HBs), and those with negative results were classified as isolated anti-HBc cases. The presence of HBV DNA in the C, S, and X gene regions was assessed by a qualitative real-time polymerase chain reaction method in all HBsAg-negative samples. OBI subjects were detected by the presence of at least one HBV genomic region.

Results: Of 540 HBsAg-negative donors, 29 (5.4%; 95% confidence interval: 3.6-7.6%) showed seroreactivity for anti-HBc, of whom 18 individuals were also seropositive for anti-HBs. All donors showed negative results for all three HBV genes regardless of their serum anti-HBc status.

Conclusion: Based on our findings, we suggest routine screening of Iranian blood donation volunteers for serum anti-HBc and anti-HBs but not HBV DNA.

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伊朗 HBsAg 阴性献血者中抗 HBc 抗体低流行率和缺乏 HBV DNA：横断面研究和文献综述。

背景：隐匿性乙型肝炎感染（OBI）是指在乙型肝炎病毒（HBV）表面抗原（HBsAg）检测呈阴性的人的血清或肝脏中存在乙型肝炎病毒（HBV）DNA。本研究旨在确定伊朗东北部马什哈德市 HBsAg 无反应献血者中 HBV 核心抗原抗体（抗 HBc）的血清阳性率和 OBI 的频率：在这项横断面研究中，2018 年 6 月至 8 月期间对 HBsAg 阴性献血者的血清样本进行了抗 HBc 检测。对抗 HBc 阳性样本进行 HBsAg（抗 HBs）抗体检测，结果为阴性的样本被归为孤立抗 HBc 病例。在所有 HBsAg 阴性样本中，采用定性实时聚合酶链反应法评估 C、S 和 X 基因区是否存在 HBV DNA。通过至少一个 HBV 基因组区域的存在来检测 OBI 受试者：结果：在 540 名 HBsAg 阴性供体中，29 人（5.4%；95% 置信区间：3.6-7.6%）出现抗 HBc 血清反应，其中 18 人的抗 HBs 血清反应也呈阳性。无论血清抗 HBc 状况如何，所有献血者的三种 HBV 基因均为阴性：根据我们的研究结果，我们建议对伊朗献血志愿者进行血清抗 HBc 和抗 HBs 的常规筛查，但不包括 HBV DNA。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Iranian Medicine 医学-医学：内科

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

3-8 weeks

期刊介绍： Aim and Scope: The Archives of Iranian Medicine (AIM) is a monthly peer-reviewed multidisciplinary medical publication. The journal welcomes contributions particularly relevant to the Middle-East region and publishes biomedical experiences and clinical investigations on prevalent diseases in the region as well as analyses of factors that may modulate the incidence, course, and management of diseases and pertinent medical problems. Manuscripts with didactic orientation and subjects exclusively of local interest will not be considered for publication.The 2016 Impact Factor of "Archives of Iranian Medicine" is 1.20.