Quinolizidine alkaloids and phomopsin A in animal feed containing lupins: co-occurrence and carry-over into veal products.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2024-08-01 Epub Date: 2024-06-10 DOI:10.1080/19440049.2024.2357706

Sofie Schryvers, Liesbeth Jacxsens, Siska Croubels, Sigrid Vonck, Bram Miserez, Jet Van De Steene, Graciele Necchi Rohers, Mia Eeckhout

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Abstract

Lupins are used in animal feed because of their excellent nutritional composition. Australian and European Lupinus angustifolius seeds are incorporated in compound feed of calves for veal production in Belgium. To investigate the co-occurrence of quinolizidine alkaloids (QAs) and phomopsin A (PHO A) in lupin seeds and lupin-containing feed, and the potential transfer to animal-derived foods, representative samples were obtained from various actors in the chain. A UHPLC-MS/MS method was validated for the simultaneous quantification of seven QAs and PHO A in relevant matrices. Results indicate highly consistent total QA (TQA) levels in Australian lupins (173 ± 24 mg/kg) (n = 25), while European samples showed a high variability (1442 ± 1497 mg/kg) (n = 15). PHO A was detected in 7 of 40 samples. Lupin-containing feed had a mean TQA content of 42 ± 28 mg/kg (n = 20). An in vivo feeding trial demonstrated the transfer of QAs to muscle and liver of calves that were fed the lupin-containing feed. Highest concentrations were found for lupanine in liver tissue samples (67 ± 46 µg/kg). PHO A concentrations were below the LOD in all feed and tissue samples. These results indicate that animal-derived foods (veal meat/liver) are a potential route for QAs to enter the food chain.

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含有羽扇豆的动物饲料中的喹诺酮类生物碱和幽门螺杆菌素 A：共同出现并迁移到小牛肉产品中。

羽扇豆因其出色的营养成分而被用于动物饲料。在比利时，澳大利亚和欧洲的羽扇豆种子被添加到小牛肉生产的配合饲料中。为了研究羽扇豆种子和含羽扇豆的饲料中同时存在的喹诺酮类生物碱（QAs）和幽门螺杆菌素 A（PHO A），以及向动物源食品转移的可能性，我们从产业链中的不同参与者处获得了具有代表性的样品。采用超高效液相色谱-质谱/质谱法对相关基质中的七种 QA 和 PHO A 进行了同时定量。结果表明，澳大利亚羽扇豆中的总 QA (TQA) 含量高度一致（173 ± 24 mg/kg）（n = 25），而欧洲样品中的总 QA (TQA) 含量差异较大（1442 ± 1497 mg/kg）（n = 15）。40 个样本中有 7 个检测到 PHO A。含羽扇豆的饲料的平均 TQA 含量为 42 ± 28 毫克/千克（n = 20）。一项体内喂养试验表明，喂养含羽扇豆饲料的犊牛的肌肉和肝脏中会转移 QA。肝脏组织样本中的羽扇豆碱浓度最高（67 ± 46 µg/kg）。所有饲料和组织样本中的 PHO A 浓度均低于检测限。这些结果表明，动物源性食品（小牛肉/肝脏）是 QA 进入食物链的潜在途径。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.