Ling Zeng, Ting Yu, Haijun Liu, Mi Li, Jin Wang, Changsheng Wang, Ping Xu
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引用次数: 0
Abstract
Purpose: Patients with obstructive sleep apnea syndrome (OSAS) frequently experience cognitive dysfunction, which may be connected to chronic intermittent hypoxia (CIH). Insulin-like growth factor-1 (IGF-1) is thought to be closely associated with cognitive function, but its role in cognitive impairment caused by OSAS is unclear. The purpose of this study was to investigate the potential protective effect of IGF-1 on cognitive impairment in OSAS rats.
Methods: Healthy male SD rats (n = 40) were randomly assigned into four groups: control group, CIH group, NS + CIH group, and IGF-1 + CIH group. All experimental rats except for those in the control group were exposed to intermittent hypoxic (IH) environments for 8 h per day over 28 days. Prior to daily exposure to IH, rats in the IGF-1 + CIH group received subcutaneous injections of IGF-1. The Morris water maze test was conducted on all experimental rats. Brain tissue testing methods included Enzyme-Linked Immunosorbent Assay, Hematoxylin and eosin staining, Immunohistochemistry, and Western blotting.
Results: The rat model of OSAS was successfully established following exposure to CIH and exhibited significant cognitive impairment. However, daily subcutaneous injections of IGF-1 partially restored the impaired cognitive function in OSAS rats. Compared with the control group, there was a significant decrease in the expression levels of IGF-1, p-IGF-IR, and SYP in the CIH group; however, these expression levels increased significantly in the IGF-I + CIH group.
Conclusion: In OSAS rats, IGF-1 enhances learning memory; this effect may be linked to increased p-IGF-1R and SYP protein production in the hippocampus.
目的：阻塞性睡眠呼吸暂停综合征（OSAS）患者经常出现认知功能障碍，这可能与慢性间歇性缺氧（CIH）有关。胰岛素样生长因子-1（IGF-1）被认为与认知功能密切相关，但其在 OSAS 引起的认知功能障碍中的作用尚不清楚。方法：将健康雄性 SD 大鼠（n = 40）随机分为四组：对照组、CIH 组、NS + CIH 组和 IGF-1 + CIH 组。除对照组外，所有实验鼠均暴露于间歇性缺氧（IH）环境中，每天 8 小时，持续 28 天。在每天暴露于 IH 之前，IGF-1 + CIH 组大鼠皮下注射 IGF-1。所有实验鼠都进行了莫里斯水迷宫测试。脑组织检测方法包括酶联免疫吸附试验、苏木精和伊红染色、免疫组织化学和 Western 印迹：结果：大鼠暴露于 CIH 后成功建立了 OSAS 模型，并表现出明显的认知障碍。然而，每天皮下注射 IGF-1 可部分恢复 OSAS 大鼠受损的认知功能。与对照组相比，CIH组IGF-1、p-IGF-IR和SYP的表达水平显著下降；但在IGF-I + CIH组，这些表达水平显著上升：结论：在 OSAS 大鼠中，IGF-1 可增强学习记忆；这种效应可能与海马中 p-IGF-1R 和 SYP 蛋白生成增加有关。
期刊介绍：
The journal Sleep and Breathing aims to reflect the state of the art in the international science and practice of sleep medicine. The journal is based on the recognition that management of sleep disorders requires a multi-disciplinary approach and diverse perspectives. The initial focus of Sleep and Breathing is on timely and original studies that collect, intervene, or otherwise inform all clinicians and scientists in medicine, dentistry and oral surgery, otolaryngology, and epidemiology on the management of the upper airway during sleep.
Furthermore, Sleep and Breathing endeavors to bring readers cutting edge information about all evolving aspects of common sleep disorders or disruptions, such as insomnia and shift work. The journal includes not only patient studies, but also studies that emphasize the principles of physiology and pathophysiology or illustrate potentially novel approaches to diagnosis and treatment. In addition, the journal features articles that describe patient-oriented and cost-benefit health outcomes research. Thus, with peer review by an international Editorial Board and prompt English-language publication, Sleep and Breathing provides rapid dissemination of clinical and clinically related scientific information. But it also does more: it is dedicated to making the most important developments in sleep disordered breathing easily accessible to clinicians who are treating sleep apnea by presenting well-chosen, well-written, and highly organized information that is useful for patient care.
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