Identification of mitophagy and ferroptosis-related hub genes associated with intracerebral haemorrhage through bioinformatics analysis.

IF 1.2 4区医学 Q2 ANTHROPOLOGY Annals of Human Biology Pub Date : 2024-02-01 Epub Date: 2024-06-12 DOI:10.1080/03014460.2024.2334719

Yan Wang, Rufeng Wang, Jianzhong Zhu, Ling Chen

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Abstract

Background: Mitophagy and ferroptosis occur in intracerebral haemorrhage (ICH) but our understanding of mitophagy and ferroptosis-related genes remains incomplete.

Aim: This study aims to identify shared ICH genes for both processes.

Methods: ICH differentially expressed mitophagy and ferroptosis-related genes (DEMFRGs) were sourced from the GEO database and literature. Enrichment analysis elucidated functions. Hub genes were selected via STRING, MCODE, and MCC algorithms in Cytoscape. miRNAs targeting hubs were predicted using miRWalk 3.0, forming a miRNA-hub gene network. Immune microenvironment variances were assessed with MCP and TIMER. Potential small molecules for ICH were forecasted via CMap database.

Results: 64 DEMFRGs and ten hub genes potentially involved in various processes like ferroptosis, TNF signalling pathway, MAPK signalling pathway, and NF-kappa B signalling pathway were discovered. Several miRNAs were identified as shared targets of hub genes. The ICH group showed increased infiltration of monocytic lineage and myeloid dendritic cells compared to the Healthy group. Ten potential small molecule drugs (e.g. Zebularine, TWS-119, CG-930) were predicted via CMap.

Conclusion: Several shared genes between mitophagy and ferroptosis potentially drive ICH progression via TNF, MAPK, and NF-kappa B pathways. These results offer valuable insights for further exploring the connection between mitophagy, ferroptosis, and ICH.

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通过生物信息学分析鉴定与脑内出血相关的有丝分裂和铁突变相关的枢纽基因。

背景：目的：本研究旨在确定这两个过程的共有 ICH 基因：方法：从 GEO 数据库和文献中获取 ICH 差异表达的有丝分裂和铁突变相关基因（DEMFRGs）。富集分析阐明了这些基因的功能。利用 miRWalk 3.0 预测了针对枢纽基因的 miRNA，形成了 miRNA 枢纽基因网络。用 MCP 和 TIMER 评估了免疫微环境差异。通过 CMap 数据库预测了治疗 ICH 的潜在小分子药物：结果：发现了 64 个 DEMFRGs 和 10 个可能参与铁变态反应、TNF 信号通路、MAPK 信号通路和 NF-kappa B 信号通路等各种过程的枢纽基因。一些 miRNA 被确定为枢纽基因的共同靶标。与健康组相比，ICH 组的单核细胞系和髓系树突状细胞浸润增加。通过CMap预测了10种潜在的小分子药物（如Zebularine、TWS-119、CG-930）：结论：有丝分裂和铁突变之间的几个共享基因可能会通过 TNF、MAPK 和 NF-kappa B 通路驱动 ICH 的进展。这些结果为进一步探索有丝分裂、铁蛋白沉积和 ICH 之间的联系提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Human Biology 生物-公共卫生、环境卫生与职业卫生

CiteScore

3.40

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： Annals of Human Biology is an international, peer-reviewed journal published six times a year in electronic format. The journal reports investigations on the nature, development and causes of human variation, embracing the disciplines of human growth and development, human genetics, physical and biological anthropology, demography, environmental physiology, ecology, epidemiology and global health and ageing research.