SNX14 inhibits autophagy via the PI3K/AKT/mTOR signaling cascade in breast cancer cells.

IF 2.9 4区生物学 Q3 CELL BIOLOGY Journal of Molecular Histology Pub Date : 2024-08-01 Epub Date: 2024-06-13 DOI:10.1007/s10735-024-10209-1

Sha Lv, Hongyan Jiang, Lingyan Yu, Yafei Zhang, Liangliang Sun, Junjun Xu

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Abstract

Background: Sorting nexin 14 (SNX14) is a member of the sorting junction protein family. Its specific roles in cancer development remain unclear. Therefore, in this study, we aimed to determine the effects and underlying mechanisms of SNX14 on autophagy of breast cancer cells to aid in the therapeutic treatment of breast cancer.

Methods: In this study, we performed in vitro experiments to determine the effect of SNX14 on breast cancer cell growth. Moreover, we used an MCF7 breast cancer tumor-bearing mouse model to confirm the effect of SNX14 on tumor cell growth in vivo. We also performed western blotting and quantitative polymerase chain reaction to identify the mechanism by which SNX14 affects breast cancer MCF7 cells.

Results: We found that SNX14 regulated the onset and progression of breast cancer by promoting the proliferation and inhibiting the autophagy of MCF7 breast cancer cells. In vivo experiments further confirmed that SNX14 knockdown inhibited the tumorigenicity and inhibited the growth of tumor cells in tumor tissues of nude mice. In addition, western blotting analysis revealed that SNX14 modulate the autophagy of MCF7 breast cancer cells via the phosphoinositide 3-kinase/protein kinase B/mechanistic target of rapamycin kinase signaling pathway.

Conclusion: Our findings indicate that SNX14 is an essential tumor-promoting factor in the development of breast cancer.

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SNX14 可通过 PI3K/AKT/mTOR 信号级联抑制乳腺癌细胞的自噬。

背景：分拣接头蛋白 14（SNX14）是分拣接头蛋白家族的成员。它在癌症发展中的具体作用尚不清楚。因此，在本研究中，我们旨在确定 SNX14 对乳腺癌细胞自噬的影响及其潜在机制，以帮助乳腺癌的治疗：在本研究中，我们进行了体外实验，以确定 SNX14 对乳腺癌细胞生长的影响。此外，我们还利用 MCF7 乳腺癌肿瘤小鼠模型证实了 SNX14 对体内肿瘤细胞生长的影响。我们还进行了 Western 印迹和定量聚合酶链反应，以确定 SNX14 对乳腺癌 MCF7 细胞的影响机制：结果：我们发现 SNX14 通过促进 MCF7 乳腺癌细胞的增殖和抑制其自噬来调控乳腺癌的发生和发展。体内实验进一步证实，敲除 SNX14 能抑制肿瘤的致瘤性，并能抑制裸鼠肿瘤组织中肿瘤细胞的生长。此外，Western印迹分析显示，SNX14通过磷酸肌醇3-激酶/蛋白激酶B/雷帕霉素激酶机制靶点信号通路调节MCF7乳腺癌细胞的自噬：我们的研究结果表明，SNX14是乳腺癌发展过程中不可或缺的肿瘤促进因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.