SPP-5 affects larval arrest via insulin signaling pathway in Caenorhabditis elegans

IF 2.9 4区生物学 Q3 CELL BIOLOGY Journal of Molecular Histology Pub Date : 2024-06-13 DOI:10.1007/s10735-024-10205-5

Guangjie Xie, Zhiyong Shao

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Abstract

Diapause is an endocrine-mediated metabolic and growth arrest state in response to unfavorable external environments. The nematode Caenorhabditis elegans can enter diapause/arrest during embryonic, larval, or adult stages when subjected to detrimental external environments. Larval stage 1 (L1) arrest happens when animals hatch without food. Previous work has shown that the insulin pathway plays a prominent role in regulating L1 arrest. However, the downstream signal molecular mechanisms and biomarkers are still missing. In this study, we showed that SaPosin-like Protein family member SPP-5 is significantly upregulated during L1 arrest, suggesting that it could act as an L1 arrest biomarker. Using RNA interference we demonstrated that spp-5 knockdown accelerated larval development, while the overexpression resulted in L1 arrest. Consistently, SPP-5 level was significantly up-regulated in the L1 arrest daf-2(e1370) mutants, and spp-5(RNAi) suppressed the daf-2(e1370) induced L1 arrest. These results suggest that SPP-5 can serve as an L1 arrest biomarker and promote the arrest probably via the insulin signaling pathway.

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SPP-5通过胰岛素信号通路影响秀丽隐杆线虫的幼虫停滞。

休眠是一种由内分泌介导的新陈代谢和生长停滞状态，以应对不利的外部环境。秀丽隐杆线虫在胚胎期、幼虫期或成虫期遇到不利的外部环境时，都会进入休眠/停滞状态。幼虫期 1（L1）停滞发生在动物孵化时没有食物的情况下。先前的研究表明，胰岛素通路在调节 L1 期停滞中起着重要作用。然而，下游信号分子机制和生物标志物仍然缺失。在这项研究中，我们发现SaPosin样蛋白家族成员SPP-5在L1停滞过程中显著上调，这表明它可以作为L1停滞的生物标志物。通过 RNA 干扰，我们证明了 spp-5 敲除会加速幼虫发育，而过表达则会导致 L1 停滞。同样，SPP-5水平在L1停滞的daf-2（e1370）突变体中显著上调，而spp-5（RNAi）抑制了daf-2（e1370）诱导的L1停滞。这些结果表明，SPP-5可作为L1停滞的生物标志物，并可能通过胰岛素信号通路促进停滞。

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.