Knockdown of type 2 orexin receptor in adult mouse testis potentiates testosterone production and germ cell proliferation

IF 3.8 3区 医学 Q2 CELL BIOLOGY Molecular and Cellular Endocrinology Pub Date : 2024-06-10 DOI:10.1016/j.mce.2024.112312
Pratikshya Sahoo , Debarshi Sarkar , Shubhangi Sharma , Arpit Verma , Suraj Kumar Naik , Vikash Prashar , Jyoti Parkash , Shio Kumar Singh
{"title":"Knockdown of type 2 orexin receptor in adult mouse testis potentiates testosterone production and germ cell proliferation","authors":"Pratikshya Sahoo ,&nbsp;Debarshi Sarkar ,&nbsp;Shubhangi Sharma ,&nbsp;Arpit Verma ,&nbsp;Suraj Kumar Naik ,&nbsp;Vikash Prashar ,&nbsp;Jyoti Parkash ,&nbsp;Shio Kumar Singh","doi":"10.1016/j.mce.2024.112312","DOIUrl":null,"url":null,"abstract":"<div><p>Orexins (OXs) are neuropeptides which regulate various physiological processes. OXs exist in two different forms, mainly orexin A (OXA) and orexin B (OXB) and their effects are mediated via OX1R and OX2R. Presence of OXB and OX2R in mouse testis is also reported. However, the role of OXB/OX2R in the male gonad remains unexplored. Herein we investigated the role of OXB/OX2R system in testicular physiology under <em>in vivo</em> and <em>ex vivo</em> conditions. Adult mice were given a single dose of bilateral intratesticular injection of siRNA targeting OX2R and were sacrificed 96 h post-injection. OX2R-knockdown potentiated serum and intratesticular testosterone levels with up-regulation in the expressions of major steroidogenic proteins. Germ cell proliferation also increased in siRNA-treated mice. Results of the <em>ex vivo</em> experiment also supported the findings of the <em>in vivo</em> study. In conclusion, OX2R may regulate testosterone production and thereby control the fine-tuning between steroidogenesis and germ cell dynamics.</p></div>","PeriodicalId":18707,"journal":{"name":"Molecular and Cellular Endocrinology","volume":"592 ","pages":"Article 112312"},"PeriodicalIF":3.8000,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and Cellular Endocrinology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0303720724001680","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Orexins (OXs) are neuropeptides which regulate various physiological processes. OXs exist in two different forms, mainly orexin A (OXA) and orexin B (OXB) and their effects are mediated via OX1R and OX2R. Presence of OXB and OX2R in mouse testis is also reported. However, the role of OXB/OX2R in the male gonad remains unexplored. Herein we investigated the role of OXB/OX2R system in testicular physiology under in vivo and ex vivo conditions. Adult mice were given a single dose of bilateral intratesticular injection of siRNA targeting OX2R and were sacrificed 96 h post-injection. OX2R-knockdown potentiated serum and intratesticular testosterone levels with up-regulation in the expressions of major steroidogenic proteins. Germ cell proliferation also increased in siRNA-treated mice. Results of the ex vivo experiment also supported the findings of the in vivo study. In conclusion, OX2R may regulate testosterone production and thereby control the fine-tuning between steroidogenesis and germ cell dynamics.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
敲除成年小鼠睾丸中的 2 型奥曲肽受体可促进睾酮生成和生殖细胞增殖。
奥曲肽是一种神经肽,可调节各种生理过程。OXs 以两种不同的形式存在,主要是 OXin A(OXA)和 OXin B(OXB),其作用通过 OX1R 和 OX2R 介导。也有报道称小鼠睾丸中存在 OXB 和 OX2R。然而,OXB/OX2R在雄性性腺中的作用仍未得到探索。在此,我们研究了体内和体外条件下 OXB/OX2R 系统在睾丸生理中的作用。成年小鼠接受单剂量双侧睾丸内注射靶向 OX2R 的 siRNA,注射后 96 小时处死。OX2R敲除可提高血清和睾丸内睾酮水平,并上调主要类固醇生成蛋白的表达。经 siRNA 处理的小鼠生殖细胞增殖也有所增加。体内外实验的结果也支持了体内研究的发现。总之,OX2R 可调节睾酮的产生,从而控制类固醇生成和生殖细胞动态之间的微调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Molecular and Cellular Endocrinology
Molecular and Cellular Endocrinology 医学-内分泌学与代谢
CiteScore
9.00
自引率
2.40%
发文量
174
审稿时长
42 days
期刊介绍: Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.
期刊最新文献
Editorial Board Stress in pregnancy alters hepatic unfolded protein responses in male adult offspring Luteal fibroblasts produce prostaglandins in response to IL1β in a MAPK-mediated manner Oridonin attenuates diabetic retinopathy progression by suppressing NLRP3 inflammasome pathway Molecular basis of photoinduced seasonal energy rheostasis in Japanese quail (Coturnix japonica)
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1