Clinical significance of lncRNA XIST expression in cholangiocarcinoma and its effect on cell migration and invasion

IF 2.6 4区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Clinics and research in hepatology and gastroenterology Pub Date : 2024-06-11 DOI:10.1016/j.clinre.2024.102398

Chenxi Li , Yifei Dong , Yichuan Zhang , Caihong Wu

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Abstract

Background

Cholangiocarcinoma is a malignant tumor that occurs in the bile duct system, and the prognosis of patients is poor. Currently, research suggests that long non-coding RNAs (lncRNAs) in the treatment and prevention of cholangiocarcinoma. This study primarily focuses on the regulation and potential mechanism of the lncRNA XIST (XIST) in cholangiocarcinoma.

Methods

The levels of XIST and miR-126–3p in cholangiocarcinoma tissues and cells were detected using real-time quantitative polymerase chain reaction (RT-qPCR). Cell transfection status, including migration and invasion, was examined via the Transwell method. The relationship between XIST and miR-126–3p was observed by dual-luciferase gene reporter assay and verified by rescue assays. Additionally, the prognostic significance of XIST in cholangiocarcinoma was determined using Kaplan-Meier and multivariate Cox regression analyses.

Results

XIST expression was increased in cholangiocarcinoma, while miR-126–3p was decreased, in both tissues and cells. The successful construction of silencing XIST was found to inhibit the count of cell migration and invasion. XIST directly targeted miR-126–3p to regulate the progression of cholangiocarcinoma.

Conclusion

XIST sponging miR-126–3p inhibited the progression of cholangiocarcinoma and improved the prognosis for patients. This finding provides new insights and opportunities for future studies on cholangiocarcinoma prognostic biomarkers.

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胆管癌中 lncRNA XIST 表达的临床意义及其对细胞迁移和侵袭的影响

背景：胆管癌是发生在胆管系统的恶性肿瘤，患者预后较差。目前，研究表明长非编码 RNA（lncRNA）可用于治疗和预防胆管癌。本研究主要关注lncRNA XIST（XIST）在胆管癌中的调控和潜在机制：方法：采用实时定量聚合酶链反应（RT-qPCR）检测胆管癌组织和细胞中 XIST 和 miR-126-3p 的水平。通过 Transwell 法检测细胞的转染状态，包括迁移和侵袭。通过双荧光素酶基因报告实验观察了 XIST 与 miR-126-3p 之间的关系，并通过挽救实验进行了验证。此外，还利用 Kaplan-Meier 和多变量 Cox 回归分析确定了 XIST 在胆管癌中的预后意义：结果：在组织和细胞中，胆管癌中的XIST增加，而miR-126-3p减少。沉默 XIST 的成功构建可抑制细胞的迁移和侵袭。XIST直接靶向miR-126-3p，调控胆管癌的进展：结论：XIST与miR-126-3p的结合抑制了胆管癌的进展，改善了患者的预后。这一发现为今后研究胆管癌预后生物标志物提供了新的见解和机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinics and research in hepatology and gastroenterology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.30

自引率

3.70%

发文量

198

审稿时长

42 days

期刊介绍： Clinics and Research in Hepatology and Gastroenterology publishes high-quality original research papers in the field of hepatology and gastroenterology. The editors put the accent on rapid communication of new research and clinical developments and so called "hot topic" issues. Following a clear Editorial line, besides original articles and case reports, each issue features editorials, commentaries and reviews. The journal encourages research and discussion between all those involved in the specialty on an international level. All articles are peer reviewed by international experts, the articles in press are online and indexed in the international databases (Current Contents, Pubmed, Scopus, Science Direct). Clinics and Research in Hepatology and Gastroenterology is a subscription journal (with optional open access), which allows you to publish your research without any cost to you (unless you proactively chose the open access option). Your article will be available to all researchers around the globe whose institution has a subscription to the journal.