Electroacupuncture alleviates diabetes-induced mechanical allodynia and downregulates bradykinin B1 receptor expression in spinal cord dorsal horn.

IF 1.6 4区医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-08-07 Epub Date: 2024-06-05 DOI:10.1097/WNR.0000000000002059

Hengyu Chi, Qunqi Hu, Xiang Li, Yurong Kang, Yu Zheng, Mingjian Jiang, Xinnan Xu, Xiaoxiang Wang, Xiaofen He

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Abstract

Objective: Diabetic neuropathic pain (DNP) is one of the most prevalent symptoms of diabetes. The alteration of proteins in the spinal cord dorsal horn (SCDH) plays a significant role in the genesis and the development of DNP. Our previous study has shown electroacupuncture could effectively relieve DNP. However, the potential mechanism inducing DNP's genesis and development remains unclear and needs further research.

Methods: This study established DNP model rats by intraperitoneally injecting a single high-dose streptozotocin; 2 Hz electroacupuncture was used to stimulate Zusanli (ST36) and Kunlun (BL60) of DNP rats daily from day 15 to day 21 after streptozotocin injection. Behavioral assay, quantitative PCR, immunofluorescence staining, and western blotting were used to study the analgesic mechanism of electroacupuncture.

Results: The bradykinin B1 receptor (B1R) mRNA, nuclear factor-κB p65 (p65), substance P, and calcitonin gene-related peptide (CGRP) protein expression were significantly enhanced in SCDH of DNP rats. The paw withdrawal threshold was increased while body weight and fasting blood glucose did not change in DNP rats after the electroacupuncture treatment. The expression of B1R, p65, substance P, and CGRP in SCDH of DNP rats was also inhibited after the electroacupuncture treatment.

Conclusion: This work suggests that the potential mechanisms inducing the allodynia of DNP rats were possibly related to the increased expression of B1R, p65, substance P, and CGRP in SCDH. Downregulating B1R, p65, substance P, and CGRP expression levels in SCDH may achieve the analgesic effect of 2 Hz electroacupuncture treatment.

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电针可减轻糖尿病引起的机械痛觉并下调脊髓背角缓激肽B1受体的表达。

目的：糖尿病神经病理性疼痛（DNP）是糖尿病最常见的症状之一。脊髓背角（SCDH）蛋白质的改变在 DNP 的发生和发展中起着重要作用。我们之前的研究表明，电针可以有效缓解 DNP。然而，诱导 DNP 发生和发展的潜在机制仍不清楚，有待进一步研究：方法：本研究通过腹腔注射大剂量链脲佐菌素，建立 DNP 模型大鼠；在注射链脲佐菌素后第 15 天至第 21 天，每天用 2 赫兹电针刺激 DNP 大鼠的足三里（ST36）和昆仑（BL60）。采用行为测定、定量 PCR、免疫荧光染色和 Western 印迹等方法研究电针的镇痛机制：结果：缓激肽 B1 受体（B1R）mRNA、核因子-κB p65（p65）、P 物质和降钙素基因相关肽（CGRP）蛋白在 DNP 大鼠 SCDH 中的表达显著增强。电针治疗后，DNP大鼠爪退缩阈值升高，体重和空腹血糖无变化。电针治疗后，DNP 大鼠 SCDH 中 B1R、p65、P 物质和 CGRP 的表达也受到抑制：结论：这项研究表明，诱导 DNP 大鼠异动症的潜在机制可能与 SCDH 中 B1R、p65、P 物质和 CGRP 的表达增加有关。降低SCDH中B1R、p65、P物质和CGRP的表达水平可能会达到2 Hz电针治疗的镇痛效果。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.