Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-06-01 DOI:10.1111/mpp.13487
Hao Sun, Malgorzata Ciska, Mongia Makki, Francisco Tenllado, Tomás Canto
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Abstract

We had previously reported that a plum pox virus (PPV)-based chimera that had its P1-HCPro bi-cistron replaced by a modified one from potato virus Y (PVY) increased its virulence in some Nicotiana benthamiana plants, after mechanical passages. This correlated with the natural acquisition of amino acid substitutions in several proteins, including in HCPro at either position 352 (Ile→Thr) or 454 (Leu→Arg), or of mutations in non-coding regions. Thr in position 352 is not found among natural potyviruses, while Arg in 454 is a reversion to the native PVY HCPro amino acid. We show here that both mutations separately contributed to the increased virulence observed in the passaged chimeras that acquired them, and that Thr in position 352 is no intragenic suppressor to a Leu in position 454, because their combined effects were cumulative. We demonstrate that Arg in position 454 improved HCPro autocatalytic cleavage, while Thr in position 352 increased its accumulation and the silencing suppression of a reporter in agropatch assays. We assessed infection by four cloned chimera variants expressing HCPro with none of the two substitutions, one of them or both, in wild-type versus DCL2/4-silenced transgenic plants. We found that during infection, the transgenic context of altered small RNAs affected the accumulation of the four HCPro variants differently and hence, also infection virulence.

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HCPro 两个氨基酸的适应性替代改变了其功能特性,从而分别提高了潜病毒嵌合体的毒力。
我们以前曾报道过,一种基于梅花痘病毒(PPV)的嵌合体,其 P1-HCPro 双组字体被马铃薯病毒 Y(PVY)的改良组字体所取代,经过机械传递后,它在一些烟草植物中的毒力增强了。这与几种蛋白质中氨基酸替代的自然获得有关,包括 HCPro 的 352 位(Ile→Thr)或 454 位(Leu→Arg),或非编码区的突变。352 位上的 Thr 在天然壶状病毒中并不存在,而 454 位上的 Arg 则是 PVY HCPro 的原生氨基酸。我们在此表明,这两个突变分别导致了获得这两个突变的传代嵌合体的毒力增强,而且 352 位的 Thr 对 454 位的 Leu 没有基因内抑制作用,因为它们的综合效应是累积性的。我们证明,454 位的 Arg 提高了 HCPro 的自催化裂解能力,而 352 位的 Thr 则增加了 HCPro 的积累,并抑制了农配试验中报告基因的沉默。我们评估了四种表达 HCPro 的克隆嵌合体变体在野生型与 DCL2/4 沉默的转基因植物中的感染情况。我们发现,在感染过程中,小 RNA 改变的转基因环境对四种 HCPro 变体的积累产生了不同的影响,因此也影响了感染的毒力。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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