Evaluation of oxidative damage and genotoxicity in populations exposed to arsenic in drinking water from Santa Fe province, Argentina

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-06-13 DOI:10.1016/j.mrgentox.2024.503787

Ana María Quiroga , Carlina L. Colussi , Lucía M. Odetti , Alicia E. Loteste , Adriana M. Paonessa , Carlos R. Mastandrea , Raúl A. Grigolato , Gisela L. Poletta , Mirna Sigrist , M. Fernanda Simoniello

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Abstract

The presence of arsenic in the environment is a public health problem. Groundwater of certain regions of Argentina contains arsenic of natural origin in concentrations that exceed the guide level recommended by World Health Organization (WHO, 10 µg/L). Pathologies derived from chronic arsenic consumption justify the planning of human biomonitoring. Hence, the aim of this study was to evaluate oxidative damage and genotoxicity and its relationship with nutritional variables in populations exposed to arsenic through drinking water in Santa Fe province, Argentina. A total of 322 participants were analyzed for arsenic in urine together with biomarkers of genotoxicity (Comet assay in blood and frequency of Micronuclei and other Nuclear Abnormalities in exfoliated buccal cells) and oxidative stress (modified Comet assay with Endonuclease III, Lipid peroxidation and antioxidant enzyme activity), as well as nutritional and biochemical variables. Results showed that 45 % of participants excreted arsenic in the urine. Consumption of water with arsenic, whether currently or previously, was associated with statistically significant increase of oxidative DNA damage and lipid peroxidation. MN in exfoliated buccal cells serve as an early biomarker of genotoxicity and showed significant differences in the current exposed group. Biochemical results indicate dyslipidemias potentially linked to dietary choices, and insufficient intake of fruits and vegetables rich in antioxidants, was also noted. This study advocates risk communication to the population, educators, and health authorities, emphasizing the need for preventive health strategies and improved food education.

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评估阿根廷圣菲省饮用水中砷暴露人群的氧化损伤和遗传毒性

砷在环境中的存在是一个公共健康问题。阿根廷某些地区的地下水中含有天然砷，其浓度超过了世界卫生组织（WHO）建议的指导水平（10 µg/L）。长期摄入砷会导致病变，因此有必要对人体进行生物监测。因此，本研究旨在评估阿根廷圣菲省通过饮用水接触砷的人群的氧化损伤和遗传毒性及其与营养变量的关系。研究人员对 322 名参与者的尿液中的砷含量、遗传毒性生物标志物（血液中的彗星试验和脱落口腔细胞中的微核和其他核异常频率）、氧化应激（内切酶 III 的改良彗星试验、脂质过氧化和抗氧化酶活性）以及营养和生化变量进行了分析。结果显示，45% 的参与者通过尿液排出砷。无论是现在还是以前饮用含砷的水，都与氧化 DNA 损伤和脂质过氧化的增加有显著的统计学联系。脱落口腔细胞中的 MN 是遗传毒性的早期生物标志物，在目前接触砷的组别中显示出显著差异。生化结果表明，血脂异常可能与膳食选择有关，富含抗氧化剂的水果和蔬菜摄入量也不足。这项研究提倡向民众、教育工作者和卫生当局进行风险交流，强调需要采取预防性健康策略和改进食品教育。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.