A novel role of the cotton calcium sensor CBL3 was involved in Verticillium wilt resistance in cotton.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-08-01 Epub Date: 2024-06-15 DOI:10.1007/s13258-024-01528-4

Shengqi Gao, Xiaoyan Hao, Guo Chen, Wenran Hu, Zhun Zhao, Wukui Shao, Jianping Li, Quansheng Huang

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Abstract

Background: Verticillium wilt, causes mainly by the soilborne pathogen Verticillium dahliae, is a devastated vascular disease resulting in huge financial losses in cotton, so research on improving V. dahliae stress tolerance in cotton is the utmost importance. Calcium as the second messenger acts as a crucial role in plant innate immunity. Cytosolic Ca²⁺during the pathogen infection is a significant increase in plant immune responses. Calcineurin B-like (CBL) proteins are widely known calcium sensors that regulate abiotic stress responses. However, the role of cotton CBLs in response to V. dahliae stress remains unclear.

Objective: To discover and utilize the gene to Verticillium wilt resistance and defense response mechanism of cotton.

Methods: Through screening the gene to Verticillium wilt resistance in cotton, four GhCBL3 copies were obtained from the current common cotton genome sequences. The protein domain and phylogenetic analyses of GhCBL3 were performed using NCBI Blast, DNAMAN, and MotifScan programs. Real-time RT-PCR was used to detect the expression of GhCBL3 gene in cotton seedlings under various stress treatments. The expression construct including GhCBL3 cDNA was transduced into Agrobacterium tumefaciens (GV3101) by heat shock method and transformed into cotton plants by Virus-Induced Gene Silencing (VIGS) method. The results of silencing of GhCBl3 on ROS accumulation and plant disease resistance in cotton plants were assessed.

Results: A member of calcineurin B-like proteins (defined as GhCBL3) in cotton was obtained. The expression of GhCBL3 was significantly induced and raised by various stressors, including dahliae, jasmonic acid (JA) and H₂O₂ stresses. Knockdown GhCBL3 in cotton by Virus-Induced Gene Silencing analysis enhanced Verticillium wilt tolerance and changed the occurrence of reactive oxygen species. Some disease-resistant genes were increased in GhCBL3-silencing cotton lines.

Conclusion: GhCBL3 may function on regulating the Verticillium dahliae stress response of plants.

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棉花钙传感器 CBL3 在棉花抗轮纹病中发挥了新的作用。

背景：轮纹枯萎病主要由土壤传播的病原体大丽轮纹菌引起，是一种毁灭性维管束病害，给棉花造成了巨大的经济损失，因此提高棉花对大丽轮纹菌胁迫的耐受性是研究的重中之重。钙作为第二信使在植物先天免疫中起着至关重要的作用。在病原体感染期间，细胞膜 Ca2+ 在植物免疫反应中显著增加。钙调素 B 样蛋白（CBL）是广为人知的钙传感器，可调节非生物胁迫反应。然而，棉花 CBL 在应对大丽花病毒胁迫中的作用仍不清楚：目的：发现并利用棉花抗轮纹病基因和防御反应机制：方法：通过对棉花抗轮纹病基因的筛选，从目前常见的棉花基因组序列中获得了 4 个 GhCBL3 拷贝。利用NCBI Blast、DNAMAN和MotifScan程序对GhCBL3进行了蛋白质结构域和系统进化分析。采用实时 RT-PCR 技术检测 GhCBL3 基因在不同胁迫处理下棉花幼苗中的表达情况。采用热休克法将包括 GhCBL3 cDNA 的表达构建体转入农杆菌（GV3101），并采用病毒诱导基因沉默（VIGS）法将其转化为棉花植株。结果表明：GhCBl3是钙调磷酸酶B的一个成员：结果：获得了棉花中钙调素 B 样蛋白的一个成员（定义为 GhCBL3）。结果：在棉花中发现了一种钙调蛋白 B 样蛋白（定义为 GhCBL3），GhCBL3 在各种胁迫下（包括大丽花、茉莉酸（JA）和 H2O2 胁迫）都能显著诱导和提高其表达。通过病毒诱导基因沉默分析敲除棉花中的 GhCBL3 增强了对轮纹病的耐受性，并改变了活性氧的发生。一些抗病基因在GhCBL3沉默的棉花品系中有所增加：结论：GhCBL3 可能具有调节植物对大丽轮枝菌胁迫反应的功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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