Development of a novel three-dimensional injection guide for trigeminal ganglia

IF 2.7 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Neuroscience Methods Pub Date : 2024-06-14 DOI:10.1016/j.jneumeth.2024.110197

Zhouqiang Wu , Yafen Zhu , Lehan Xu , Wenli Lai , Xuepeng Chen , Hu Long

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Abstract

Background

Trigeminal ganglion (TG) plays an important role in the process of orthodontic pain. It’s necessary to design an accurate, precise and minimally invasive trigeminal ganglion injection guide plate to study TG.

Methods

Micro-CT was used to obtain the Dicom format data, and three-dimensional (3D) software (mimics and magics23.03) was used to reconstruct 3D head models. Design and modifications of the TG injection guide plate were performed in Magic 23.03 software, and the guide plate was produced by a 3D stereolithography printer. X-ray, micro-CT, Evans blue, and virus transduction were used to demonstrate the accuracy of the guide-assisted injection. Pain levels were evaluated after using the injection guide by a bite force test and Von Frey test.

Results

X-ray and micro-CT tests confirmed that the injection needle reached the bilateral trigeminal ganglia fossa. The Evans blue test and virus transduction proved that the injected drug could be accurately injected into the bilateral trigeminal ganglion and the lentivirus could be successfully transfected. The percentage of accurate injection was 10/10 (bilateral trigeminal ganglia). Orofacial pain induced by the trigeminal ganglion injection was mild and returned to baseline within seven days.

Conclusion

The injection guide described in this study is viable and reliable for the delivery of drugs and virus transduction into the trigeminal ganglia.

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开发新型三叉神经节三维注射指南。

背景：三叉神经节（TG）在正畸疼痛过程中扮演着重要角色。有必要设计一种准确、精确、微创的三叉神经节注射导板来研究三叉神经节：方法：使用 Micro-CT 获取 Dicom 格式数据，并使用三维（3D）软件（mimics 和 magics23.03）重建三维头部模型。在 Magic 23.03 软件中对 TG 注射导板进行了设计和修改，并通过三维立体光刻打印机制作了导板。使用 X 光、显微 CT、伊文思蓝和病毒转导来证明导板辅助注射的准确性。通过咬合力测试和 Von Frey 测试评估了使用注射导板后的疼痛程度：X光和显微 CT 检测证实注射针到达了双侧三叉神经节窝。埃文斯蓝试验和病毒转导证明，注射药物能准确注入双侧三叉神经节，慢病毒也能成功转导。准确注射率为 10/10（双侧三叉神经节）。三叉神经节注射引起的口面部疼痛轻微，并在七天内恢复到基线水平：结论：本研究中描述的注射指南在向三叉神经节输送药物和病毒转导方面是可行和可靠的。

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来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.