lncRNA-gene network analysis reveals the effects of early maternal nutrition on mineral homeostasis and energy metabolism in the fetal liver transcriptome of beef heifers

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nutritional Biochemistry Pub Date : 2024-06-13 DOI:10.1016/j.jnutbio.2024.109691
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Abstract

Maternal nutrition during pregnancy influences fetal development; however, the regulatory markers of fetal programming across different gestational phases remain underexplored in livestock models. Herein, we investigated the regulatory role of long non-coding RNAs (lncRNAs) on fetal liver gene expression, the impacts of maternal vitamin and mineral supplementation, and the rate of maternal body weight gain during the periconceptual period. To this end, crossbred Angus heifers (n=31) were randomly assigned to a 2×2 factorial design to evaluate the main effects of the rate of weight gain (low gain [LG, avg. daily gain of 0.28 kg/day] vs. moderate gain [MG, avg. daily gain of 0.79 kg/day]) and vitamins and minerals supplementation (VTM vs. NoVTM). On day 83±0.27 of gestation, fetuses were collected for morphometric measurements, and fetal liver was collected for transcriptomic and mineral analyses. The maternal diet significantly affected fetal liver development and mineral reserves. Using an RNA-Seq approach, we identified 320 unique differentially expressed genes (DEGs) across all six comparisons (FDR <0.05). Furthermore, lncRNAs were predicted through the FEELnc pipeline, revealing 99 unique differentially expressed lncRNAs (DELs). The over-represented pathways and biological processes (BPs) were associated with energy metabolism, Wnt signaling, CoA carboxylase activity, and fatty acid metabolism. The DEL-regulated BPs were associated with metal ion transport, pyrimidine metabolism, and classical energy metabolism-related glycolytic, gluconeogenic, and TCA cycle pathways. Our findings suggest that lncRNAs regulate mineral homeostasis- and energy metabolism-related gene networks in the fetal liver in response to early maternal nutrition.

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lncRNA-基因网络分析揭示了早期母体营养对肉用小母牛胎儿肝脏转录组中矿物质平衡和能量代谢的影响。
孕期母体营养影响胎儿发育;然而,在家畜模型中,不同妊娠阶段胎儿程序的调控标志物仍未得到充分探索。在此,我们研究了长非编码 RNA(lncRNA)对胎儿肝脏基因表达的调控作用、母体维生素和矿物质补充的影响以及围妊娠期母体体重增加的速度。为此,将杂交安格斯小母牛(n = 31)随机分配到2 × 2因子设计中,以评估增重率(低增重[LG,平均日增重0.28千克/天]与中增重[MG,平均日增重0.79千克/天])以及维生素和矿物质补充(VTM与无VTM)的主要影响。在妊娠第 83 ± 0.27 天,采集胎儿进行形态测量,并采集胎儿肝脏进行转录组和矿物质分析。母体饮食对胎儿肝脏发育和矿物质储备有明显影响。通过RNA-Seq方法,我们在所有六项比较中发现了320个独特的差异表达基因(DEGs)(FDR < 0.05)。此外,我们还通过 FEELnc 管道预测了 lncRNA,发现了 99 个独特的差异表达 lncRNA(DEL)。高表达途径和生物过程(BPs)与能量代谢、Wnt 信号转导、CoA 羧化酶活性和脂肪酸代谢有关。受DEL调控的生物过程与金属离子转运、嘧啶代谢以及经典的能量代谢相关的糖酵解、糖醛酸生成和TCA循环途径有关。我们的研究结果表明,lncRNA能调节胎儿肝脏中与矿物质平衡和能量代谢相关的基因网络,从而对母体的早期营养做出反应。
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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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