Adaptation and validation of a light microscope for use in energy insecure settings: a proof-of-concept study

IF 1.7 4区 生物学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of microbiological methods Pub Date : 2024-06-14 DOI:10.1016/j.mimet.2024.106974
Nina Bühler , Diana Velten , Philipp Jung , Sophie E. Müller , Sören L. Becker , Sakib Burza , Sophie Schneitler
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Abstract

Microscopy as a basic diagnostic method cannot be used everywhere globally. Validity of slide reading was tested on torch-modified microscopes. Experienced microscopists handled the modification without prior standard-adaptation. In contrast, microscopist-trainees required more detailed instructions to get acquainted with this new technique. The overall results encourage further, setting-specific validation.

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在能源不安全的环境中使用光学显微镜的适应性和验证:概念验证研究。
显微镜作为一种基本诊断方法,不可能在全球任何地方使用。在经过火炬改装的显微镜上测试了读片的有效性。经验丰富的显微镜操作员无需事先适应标准即可完成改装。相比之下,显微镜学徒需要更详细的指导才能熟悉这项新技术。总体结果鼓励进一步针对具体环境进行验证。
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来源期刊
Journal of microbiological methods
Journal of microbiological methods 生物-生化研究方法
CiteScore
4.30
自引率
4.50%
发文量
151
审稿时长
29 days
期刊介绍: The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.
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