Antidiabetic and antioxidant activity of Clitoria ternatea flower extracts and fractions on blood glucose and MDA in rats induced by alloxan

Abstract

Background: Clitoria ternatea, rich in anthocyanins with antioxidant properties, can help prevent oxidative stress and reduce complications associated with diabetes mellitus (DM). Objective:  To investigate the antidiabetic and antioxidant properties of the ethanol extract and ethyl acetate fraction of Clitoria ternatea in rats induced by Alloxan. Method: Ethanol extract from Clitoria ternatea was fractionated with ethyl acetate and then screened for phytochemicals using TLC. Antidiabetic and antioxidant activities were evaluated on alloxan-induced hyperglycemic rats at 150 mg/kgBW and 300 mg/kgBW, respectively. Result: The Clitoria ternatea flower's ethanol extract and ethyl acetate fraction had a flavonoid content of 68.003±0.366 mg QE/g and 78.767±0.262 mg QE/g, respectively. The ethyl acetate fraction, administered at 300 mg/kg BW, showed a 45.5986 mg/dl reduction in blood glucose levels, not significantly different from the positive control (acarbose (p = 0.14). The ethyl acetate fraction showed a significant reduction of 76.5% in MDA levels, similar to quercetin as a positive control (p = 0.275). Conclusion: Clitoria ternatea showed antidiabetic and antioxidant properties in alloxan-induced rats.