Protective Effects of Long Double-Stranded RNA with Different CpG Motifs against Miamiensis avidus and Viral Hemorrhagic Septicemia Virus (VHSV) Infections in Olive Flounder (Paralichthys olivaceus)

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-06-13 DOI:10.3390/fishes9060227

H. Choi, Da-Yeon Choi, Jung-Jin Park, Hye Jin Jung, Bo-Seong Kim, Sung-Chul Hong, Jun-Hwan Kim, Yue Jai Kang

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Abstract

The South Korean aquaculture industry has incurred considerable production losses due to various infectious diseases. Artificially synthesized polyinosinic–polycytidylic acid (poly I:C), structurally similar to double-stranded RNA (dsRNA) and cytidine–phosphate–guanosine oligodeoxynucleotides, can enhance immune responses and protect against diseases. Here, we investigated dsRNA molecules with different cytidine–phosphate–guanosine (CpG) motifs (dsRNA-CpGMix) as fused agents to treat Miamiensis avidus and viral hemorrhagic septicemia virus (VHSV) infection in olive flounders. We further investigated the efficacy of specific sequence motifs in dsRNA in modulating immunostimulatory effects. Fish treated with poly I:C or dsRNA-CpGMix exhibited higher survival rates than the control group. Olive flounder leukocytes stimulated with poly I:C or dsRNA-CpGMix showed increased scuticocidal activity in the presence of inactivated immune sera. dsRNA with CpG motif sequences induced higher resistance against M. avidus and VHSV infections than dsRNA without CpG motif sequences, and the dsRNA-CpGMix group showed upregulated ISG15 or Mx compared to the dsRNA-GFP group. Thus, dsRNA containing CpG motifs can be used as effective immunostimulants to enhance resistance against viral and parasitic diseases in olive flounder. The specific sequences of the CpG motifs in dsRNA may be important for enhancing immune responses and resistance against M. avidus and VHSV infections in olive flounders.

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具有不同 CpG 位点的长双链 RNA 对橄榄鲽（Paralichthys olivaceus）感染迈阿密鳗鲡病毒（Miamiensis avidus）和病毒性出血性败血症病毒（VHSV）的保护作用

由于各种传染性疾病，韩国水产养殖业遭受了巨大的生产损失。人工合成的聚肌苷酸-聚胞苷酸（poly I:C）在结构上类似于双链 RNA（dsRNA）和胞苷-磷酸鸟苷寡脱氧核苷酸，可以增强免疫反应，预防疾病。在这里，我们研究了具有不同胞苷-磷酸鸟苷（CpG）基序的dsRNA分子（dsRNA-CpGMix），并将其作为融合剂来治疗迈阿密鲷和橄榄鲽的病毒性出血性败血症病毒（VHSV）感染。我们进一步研究了dsRNA中特定序列基序在调节免疫刺激效应方面的功效。经 poly I:C 或 dsRNA-CpGMix 处理的鱼类存活率高于对照组。与不含 CpG 基序的 dsRNA 相比，含有 CpG 基序的 dsRNA 能诱导更高的抵抗 M. avidus 和 VHSV 感染的能力；与 dsRNA-GFP 组相比，dsRNA-CpGMix 组显示出 ISG15 或 Mx 的上调。因此，含有CpG基序的dsRNA可作为有效的免疫刺激剂，增强橄榄鲆对病毒和寄生虫病的抵抗力。dsRNA中CpG基序的特定序列可能对增强橄榄笛鲷的免疫反应和对M. avidus和VHSV感染的抵抗力非常重要。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.