Muscle fiber satellite cells are located at a greater distance from capillaries in patients with COPD compared with healthy controls

M. W. Betz, J. de Brandt, T. Aussieker, A. Monsegue, D. Houtvast, S. Gehlert, L. Verdijk, Luc J. C. van Loon, H. Gosker, Ramon Langen, Wim Derave, Chris Burtin, M. Spruit, T. Snijders
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Abstract

Chronic obstructive pulmonary disease (COPD) is a disease characterized by skeletal muscle dysfunction. A spatial relationship exists between satellite cells and muscle fiber capillaries, which has been suggested to be of major importance for satellite cell function. In the present study we compared the spatial relationship between satellite cells and capillaries in patients with COPD and age-matched healthy older adults.Muscle biopsies were obtained from thevastus lateralisof n=18 patients with COPD (8f/10 m, 66±5 y, mild to severe airflow obstruction) and n=18 age-, gender- and BMI-matched healthy control adults (8f/10 m, 68±5 y). Immunohistochemistry was used to assess type I/II muscle fiber size, distribution, myonuclear content, satellite cell number, and fiber capillarization. In addition, type I/II muscle fiber satellite cell distance to its nearest capillary was assessed.The percentage of type II muscle fibers was significantly greater in patients with COPD (62±10%) compared with control (50±12%, p<0.05). Muscle fiber capillarization was significantly lower in patients with COPD compared with controls (p<0.05). Whereas satellite cell content was not different between groups, type I and type II satellite cell distance to its nearest capillary was significantly greater in patients with COPD (type I: 21.3±4.8 and type II: 26.7±9.3 µm) compared with controls (type I: 16.1±3.5 and type II: 22.7±5.8 µm; p<0.05).Satellite cells are located at a greater distance from their nearest capillary in patients with COPD compared with age-matched controls. This increased distance could play a role in impaired satellite cell function in patients with COPD.
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与健康对照组相比,慢性阻塞性肺病患者的肌肉纤维卫星细胞与毛细血管的距离更远
慢性阻塞性肺病(COPD)是一种以骨骼肌功能障碍为特征的疾病。卫星细胞和肌纤维毛细血管之间存在空间关系,这被认为对卫星细胞的功能至关重要。本研究中,我们比较了慢性阻塞性肺病患者和年龄匹配的健康老年人的卫星细胞与毛细血管之间的空间关系。免疫组化用于评估 I/II 型肌纤维的大小、分布、肌核含量、卫星细胞数量和纤维毛细血管化。与对照组(50±12%,P<0.05)相比,慢性阻塞性肺病患者的II型肌纤维比例明显增加(62±10%)。与对照组相比,慢性阻塞性肺病患者的肌纤维毛细血管化程度明显降低(P<0.05)。虽然卫星细胞含量在组间无差异,但与对照组(I 型:16.1±3.5 µm,II 型:22.7±5.8 µm;p<0.05)相比,慢性阻塞性肺病患者的 I 型和 II 型卫星细胞与其最近毛细血管的距离明显更大(I 型:21.3±4.8 µm,II 型:26.7±9.3 µm)。与年龄匹配的对照组相比,慢性阻塞性肺病患者的卫星细胞与最近的毛细血管的距离更远,这种距离的增加可能是慢性阻塞性肺病患者卫星细胞功能受损的原因之一。
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