Development of vitamin K analysis method using column switching high-performance liquid chromatography method and analysis results of various food items for vitamin K content

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Analytical Methods Pub Date : 2024-06-11 DOI:10.1007/s12161-024-02643-4

Rina Tanaka, Ryusuke Tanaka

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Abstract

In this study, we developed a column-switching high-performance liquid chromatography (HPLC) method with fluorescence detection for the analysis of vitamin K. Column-switching is accomplished by changing the direction of flow using a switching valve with a set time program. Using this method, three vitamin K, phylloquinone (PK), menaquinone-4 (MK-4), and menaquinone-7 (MK-7), were separated and identified with high sensitivity, and impurities were eliminated. This method was used to determine the vitamin K content in meat, fish meat, snails, bivalves, sea urchins, seaweeds, vegetables, tea, soy products, milk products, and supplements. The results showed that chicken showed the highest content of MK-4 (15.35 ± 0.35 μg/100 g), matcha showed the highest content of PK (3069.66±80.10 μg/100 g), and dried natto showed the highest content of MK-7 (3997.57±79.42 μg/100 g). This method can also be used to analyze vitamin K in supplements and pharmaceuticals. The results of this study revealed that different manufacturers add different types of vitamin K to their commercial supplements and infant formulas. The developed method provides highly reproducible and quantitative results and allows for the rapid analysis of the three vitamin K types. Thus, the method developed in this study may aid the sequential analysis of vitamin K in different samples to assess food nutrients.

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利用柱切换高效液相色谱法开发维生素 K 分析方法及各种食品的维生素 K 含量分析结果

本研究建立了一种柱切换高效液相色谱(HPLC)荧光检测法来分析维生素K。利用这种方法，可以高灵敏度地分离和鉴定三种维生素 K，即植物醌（PK）、脑醌-4（MK-4）和脑醌-7（MK-7），并剔除杂质。该方法用于测定肉类、鱼肉、螺类、双壳贝类、海胆、海藻、蔬菜、茶叶、豆制品、奶制品和营养补充剂中维生素 K 的含量。结果表明，鸡肉中的 MK-4 含量最高（15.35 ± 0.35 μg/100 g），抹茶中的 PK 含量最高（3069.66±80.10 μg/100 g），纳豆干中的 MK-7 含量最高（3997.57±79.42 μg/100 g）。该方法也可用于分析保健品和药品中的维生素 K。研究结果表明，不同生产商在其商业补充剂和婴儿配方奶粉中添加了不同类型的维生素 K。所开发的方法可提供高度重现性和定量的结果，并可对三种维生素 K 进行快速分析。因此，本研究开发的方法可帮助对不同样品中的维生素 K 进行连续分析，以评估食品营养成分。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.