Biosynthesis of Zinc Oxide Nanoparticles and effect on seed germination of wheat (Triticum aestivum) in vitro

Mohammed Jasim Ali, Ahmed S Mohmed, Rafid Ahmed Abbas Al-khaldy
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Abstract

Zinc Oxide nanoparticles (ZnO NPs) are considered an effective factor in plant growth due to their high absorption because of their small size and effect on the plant's physiological system. This study included the biosynthesis of zinc nanoparticles by plant extract from the leaves of the Myrtus communis L. and used the following analyses ultraviolet- X-ray diffraction, Fourier transform infrared (FITR), and visible (UV-vis) spectroscopy (XRD). Using field emission scanning electron microscopy (FESEM), it was possible to analyze the ZnO NPs and determine how their concentration affected the germination rate and several other aspects of the growth of wheat Triticum aestivum seeds in vitro. The study results showed that a distinctive absorption peak at 366 nm caused by surface plasma resonance visible in the UV-Vis spectroscopy. A FESEM revealed that ZnO NPs were semi-spherical and had an average diameter of 30 nm.. The results also revealed the effectiveness of the concentration of 100 ppm in the germination percentage of wheat seeds which reached the mean of 59.17% compared with 24.17 % in the control treatment, While the length of plumule and radicle recorded 5.767 cm and 3.400 cm respectively at the same concentration. The study suggests found the effectiveness of biosynthetic zinc nanoparticles in the germination characteristics of wheat seeds.
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氧化锌纳米粒子的生物合成及其对小麦(Triticum aestivum)种子萌发的体外影响
纳米氧化锌颗粒(ZnO NPs)因其体积小、吸收率高以及对植物生理系统的影响而被认为是植物生长的有效因素。本研究包括通过从桃金娘叶片中提取植物提取物来生物合成纳米锌颗粒,并使用了以下分析方法:紫外-X 射线衍射、傅立叶变换红外(FITR)和可见光(紫外-可见)光谱(XRD)。利用场发射扫描电子显微镜(FESEM),可以对氧化锌氮氧化物进行分析,并确定其浓度如何影响小麦种子的发芽率和体外生长的其他几个方面。研究结果表明,在紫外可见光谱中,由表面等离子体共振引起的波长为 366 纳米的吸收峰清晰可见。FESEM 显示 ZnO NPs 呈半球形,平均直径为 30 nm。研究结果还显示,浓度为 100 ppm 的氧化锌纳米粒子对小麦种子发芽率的影响达到了 59.17%,而对照组的平均发芽率为 24.17%;在相同浓度下,小麦种子的羽叶和胚根长度分别为 5.767 厘米和 3.400 厘米。研究结果表明,生物合成纳米锌粒子对小麦种子的萌发特性有显著效果。
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