DNA Barcoding Revealed Mislabeling of Imported Seafood Products in Thailand

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-06-05 DOI:10.3390/fishes9060215

Deep Nithun Senathipathi, Sottawat Benjakul, Phutthipong Sukkapat, M. Detcharoen, Gururaj Moorthy, Jirakrit Saetang

{"title":"DNA Barcoding Revealed Mislabeling of Imported Seafood Products in Thailand","authors":"Deep Nithun Senathipathi, Sottawat Benjakul, Phutthipong Sukkapat, M. Detcharoen, Gururaj Moorthy, Jirakrit Saetang","doi":"10.3390/fishes9060215","DOIUrl":null,"url":null,"abstract":"Seafood mislabeling threatens customer rights and causes economic loss worldwide. The information on seafood misrepresentation in Thailand is still lacking, and the investigation and monitoring program must be well established. This study investigated the mislabeling status of imported seafood in Thailand using the DNA barcoding technique. A total of 45 imported seafood products from five distributors were included. Scientific, common, local, and market names of seafood samples were obtained from FAO and Fishbase databases. DNA was extracted, and PCR was performed using a universal primer targeting the COI gene. Species of each sample were identified with over 98% similarity based on COI sequence analysis. DNA sequence revealed 11 mislabeled samples. Among substituted species, Pangasianodon hypophthalmus and Thunnus maccoyii were found to be endangered species according to IUCN status. Products obtained from Brand-C showed the highest mislabeling rate (42.85%). The phylogenetic analysis adopted with the TIM2+F+I+G4 model showed the sequenced DNA similar to the NCBI database reference sequence. Overall, mislabeled products of imported seafood were found at the rate of 24.44%, suggesting that strict surveillance for seafood substitution should be implemented in Thailand.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"41 8","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/fishes9060215","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Seafood mislabeling threatens customer rights and causes economic loss worldwide. The information on seafood misrepresentation in Thailand is still lacking, and the investigation and monitoring program must be well established. This study investigated the mislabeling status of imported seafood in Thailand using the DNA barcoding technique. A total of 45 imported seafood products from five distributors were included. Scientific, common, local, and market names of seafood samples were obtained from FAO and Fishbase databases. DNA was extracted, and PCR was performed using a universal primer targeting the COI gene. Species of each sample were identified with over 98% similarity based on COI sequence analysis. DNA sequence revealed 11 mislabeled samples. Among substituted species, Pangasianodon hypophthalmus and Thunnus maccoyii were found to be endangered species according to IUCN status. Products obtained from Brand-C showed the highest mislabeling rate (42.85%). The phylogenetic analysis adopted with the TIM2+F+I+G4 model showed the sequenced DNA similar to the NCBI database reference sequence. Overall, mislabeled products of imported seafood were found at the rate of 24.44%, suggesting that strict surveillance for seafood substitution should be implemented in Thailand.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

DNA 条形码揭示了泰国进口海鲜产品的误标问题

海鲜标签不实威胁着消费者的权益，并在全球范围内造成经济损失。泰国目前仍缺乏有关海产品虚假标注的信息，因此必须建立完善的调查和监测计划。本研究利用 DNA 条形码技术调查了泰国进口海产品的标签虚假情况。共有来自 5 个经销商的 45 种进口海产品被纳入研究范围。海鲜样本的科学名称、通用名称、当地名称和市场名称均来自粮农组织和 Fishbase 数据库。提取 DNA 并使用针对 COI 基因的通用引物进行 PCR。根据 COI 序列分析，每个样本的物种相似度超过 98%。DNA 序列发现了 11 个错误标记的样本。在被替换的物种中，Pangasianodon hypophthalmus 和 Thunnus maccoyii 根据 IUCN 状态被认定为濒危物种。品牌 C 中的产品错误标签率最高（42.85%）。采用TIM2+F+I+G4模型进行的系统进化分析表明，测序DNA与NCBI数据库参考序列相似。总体而言，进口海产品的错误标签率为 24.44%，这表明泰国应对海产品替代品实施严格监控。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.