A new trypsin inhibitor from Centrosema plumieri effective against digestive proteases from Tribolium castaneum, an eco-friendly alternative

IF 1.4 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Protein expression and purification Pub Date : 2024-06-17 DOI:10.1016/j.pep.2024.106534

Cláudio Wilian Victor dos Santos , Antônio Thomás Da Silva , Andrea Carla de Almeida Barros , Josiel Santos do Nascimento , Luciano Aparecido Meireles Grillo , Francis Soares Gomes , Hugo Juarez Vieira Pereira

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Abstract

Tribolium castaneum, also known as the red flour beetle, is a polyphagous pest that seriously damages agricultural products, including stored and processed grains. Researchers have aimed to discover alternative pest control mechanisms that are less harmful to the ecosystem than those currently used. We conduct the purification and characterization of a protease inhibitor from C. plumieri seeds and an in vitro evaluation of its insecticidal potential against the insect pest T. castaneum. The trypsin inhibitor was isolated from C. plumieri seeds in a single-step DEAE-Sepharose column chromatography and had a molecular mass of 50 kDA. When analyzed for interaction with different proteolytic enzymes, the inhibitor exhibited specificity against trypsin and no activity against other serine proteases such as chymotrypsin and elastase-2. The isolated inhibitor was able to inhibit digestive enzymes of T. castaneum from extracts of the intestine of this insect. Therefore, we conclude that the new protease inhibitor, specific in tryptic inhibition, of protein nature from the seeds of C. plumieri was effective in inhibiting the digestive enzymes of T. castaneum and is a promising candidate in the ecological control of pests.

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一种来自 Centrosema plumieri 的新型胰蛋白酶抑制剂，可有效抑制蓖麻毛虫的消化蛋白酶，是一种环保型替代品。

Tribolium castaneum，又称红面粉甲虫，是一种多食性害虫，严重破坏农产品，包括储存和加工谷物。研究人员一直致力于发现比目前使用的害虫控制机制对生态系统危害更小的替代性害虫控制机制。我们从 C. plumieri 种子中纯化并鉴定了一种蛋白酶抑制剂，并对其对害虫 T. castaneum 的杀虫潜力进行了体外评估。通过一步 DEAE-Sepharose 柱色谱法从 C. plumieri 种子中分离出了胰蛋白酶抑制剂，其分子质量为 50 kDA。在分析与不同蛋白水解酶的相互作用时，该抑制剂表现出对胰蛋白酶的特异性，而对其他丝氨酸蛋白酶（如糜蛋白酶和弹性蛋白酶-2）没有活性。分离出的抑制剂能够抑制从蓖麻蝇肠道提取物中提取的蓖麻蝇消化酶。因此，我们得出结论，从 C. plumieri 种子中分离出的具有蛋白质性质的新型蛋白酶抑制剂（对胰蛋白酶有特异性抑制作用）能有效抑制 T. castaneum 的消化酶，是一种有希望用于害虫生态控制的候选物质。

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来源期刊

Protein expression and purification 生物-生化研究方法

CiteScore

3.70

自引率

6.20%

发文量

120

审稿时长

32 days

期刊介绍： Protein Expression and Purification is an international journal providing a forum for the dissemination of new information on protein expression, extraction, purification, characterization, and/or applications using conventional biochemical and/or modern molecular biological approaches and methods, which are of broad interest to the field. The journal does not typically publish repetitive examples of protein expression and purification involving standard, well-established, methods. However, exceptions might include studies on important and/or difficult to express and/or purify proteins and/or studies that include extensive protein characterization, which provide new, previously unpublished information.