{"title":"Mitochondria-Targetable Near-Infrared Fluorescent Probe for Visualization of Hydrogen Peroxide in Lung Injury, Liver Injury, and Tumor Models","authors":"Qi Zan, Kunyi Zhao, Ruijin Li, Yongming Yang, Xihua Yang*, Wenzhong Li, Gangli Zhang, Chuan Dong, Shaomin Shuang* and Li Fan*, ","doi":"10.1021/acs.analchem.3c05479","DOIUrl":null,"url":null,"abstract":"<p >Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) overexpressed in mitochondria has been regarded as a key biomarker in the pathological processes of various diseases. However, there is currently a lack of suitable mitochondria-targetable near-infrared (NIR) probes for the visualization of H<sub>2</sub>O<sub>2</sub> in multiple diseases, such as PM<sub>2.5</sub> exposure-induced lung injury, hepatic ischemia-reperfusion injury (HIRI), nonalcoholic fatty liver (NAFL), hepatic fibrosis (HF), and malignant tumor tissues containing clinical cancer patient samples. Herein, we conceived a novel NIR fluorescent probe (<b>HCy-H</b><sub><b>2</b></sub><b>O</b><sub><b>2</b></sub>) by introducing pentafluorobenzenesulfonyl as a H<sub>2</sub>O<sub>2</sub> sensing unit into the NIR hemicyanine platform. <b>HCy-H</b><sub><b>2</b></sub><b>O</b><sub><b>2</b></sub> exhibits good sensitivity and selectivity toward H<sub>2</sub>O<sub>2</sub>, accompanied by a remarkable “turn-on” fluorescence signal at 720 nm. Meanwhile, <b>HCy-H</b><sub><b>2</b></sub><b>O</b><sub><b>2</b></sub> has stable mitochondria-targetable ability and permits monitoring of the up-generated H<sub>2</sub>O<sub>2</sub> level during mitophagy. Furthermore, using <b>HCy-H</b><sub><b>2</b></sub><b>O</b><sub><b>2</b></sub>, we have successfully observed an overproduced mitochondrial H<sub>2</sub>O<sub>2</sub> in ambient PM<sub>2.5</sub> exposure-induced lung injury, HIRI, NAFL, and HF models through NIR fluorescence imaging. Significantly, the visualization of H<sub>2</sub>O<sub>2</sub> has been achieved in both tumor-bear mice as well as surgical specimens of cancer patients, making <b>HCy-H</b><sub><b>2</b></sub><b>O</b><sub><b>2</b></sub> a promising tool for cancer diagnosis and imaging-guided surgery.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":6.7000,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.analchem.3c05479","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Hydrogen peroxide (H2O2) overexpressed in mitochondria has been regarded as a key biomarker in the pathological processes of various diseases. However, there is currently a lack of suitable mitochondria-targetable near-infrared (NIR) probes for the visualization of H2O2 in multiple diseases, such as PM2.5 exposure-induced lung injury, hepatic ischemia-reperfusion injury (HIRI), nonalcoholic fatty liver (NAFL), hepatic fibrosis (HF), and malignant tumor tissues containing clinical cancer patient samples. Herein, we conceived a novel NIR fluorescent probe (HCy-H2O2) by introducing pentafluorobenzenesulfonyl as a H2O2 sensing unit into the NIR hemicyanine platform. HCy-H2O2 exhibits good sensitivity and selectivity toward H2O2, accompanied by a remarkable “turn-on” fluorescence signal at 720 nm. Meanwhile, HCy-H2O2 has stable mitochondria-targetable ability and permits monitoring of the up-generated H2O2 level during mitophagy. Furthermore, using HCy-H2O2, we have successfully observed an overproduced mitochondrial H2O2 in ambient PM2.5 exposure-induced lung injury, HIRI, NAFL, and HF models through NIR fluorescence imaging. Significantly, the visualization of H2O2 has been achieved in both tumor-bear mice as well as surgical specimens of cancer patients, making HCy-H2O2 a promising tool for cancer diagnosis and imaging-guided surgery.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.