The Effects of Supplementation of the Freezing Extender with Silymarin on the Quality Parameters of Frozen-Thawed Arabian Stallion Sperm: A Preliminary Evaluation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-06-21 DOI:10.1089/bio.2023.0131

Farzaneh Khorsand, Hossein Hamali, Babak Qasemi-Panahi, Mohammadreza Tohidkia

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Abstract

This study evaluated the effects of supplementation of the freezing extender with different concentrations of silymarin on the quality of frozen-thawed Arabian stallion spermatozoa. Semen samples from three stallions (1, 2, and 3) were suspended in the freezing extender without or with silymarin (0, 25 μg/mL, 50 μg/mL, 75 μg/mL, and 100 μg/mL) and cryopreserved in 0.5 mL straws. After 1 month of storage, the frozen semen samples in straws were thawed and evaluated in terms of viability, mitochondrial membrane potential, kinematic parameters, total and progressive motility, plasma membrane integrity, lipid peroxidation, and DNA fragmentation. The findings indicated that 25-100 μg/mL of silymarin significantly improved viability and mitochondrial membrane potential while reducing the stallion sperm lipid peroxidation, DNA fragmentation, and apoptosis compared with the control group (p < 0.05). Silymarin concentrations of 75 μg/mL and 100 μg/mL significantly increased progressive motility and plasma membrane integrity (p < 0.05). Based on our findings, it can be inferred that silymarin exhibited a dose-dependent enhancement in the frozen-thawed Arabian stallion sperm quality. The most favorable outcomes were observed when 100 μg/mL silymarin was used.

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补充水飞蓟素冷冻扩展剂对冷冻解冻阿拉伯种马精子质量参数的影响：初步评估

本研究评估了在冷冻扩展剂中添加不同浓度的水飞蓟素对冷冻解冻的阿拉伯种公马精子质量的影响。将三头种公马（1、2 和 3）的精液样本悬浮在不含或添加了水飞蓟素（0、25 μg/mL、50 μg/mL、75 μg/mL和 100 μg/mL）的冷冻延伸剂中，并冷冻保存在 0.5 mL 吸管中。储存 1 个月后，解冻吸管中的冷冻精液样本，并从活力、线粒体膜电位、运动参数、总运动和渐进运动、质膜完整性、脂质过氧化和 DNA 断裂等方面进行评估。研究结果表明，与对照组相比，25-100 μg/mL 水飞蓟素能显著提高种公马精子的活力和线粒体膜电位，同时减少脂质过氧化、DNA 断裂和凋亡（p < 0.05）。浓度为 75 μg/mL 和 100 μg/mL 的水飞蓟素能显著提高精子的运动能力和质膜完整性（p < 0.05）。根据我们的研究结果，可以推断水飞蓟素对冷冻解冻的阿拉伯种公马精子质量的提高具有剂量依赖性。当使用 100 μg/mL 水飞蓟素时，观察到了最有利的结果。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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