WNT16 as a promising biomarker for systemic lupus erythematosus and its role in regulating cell proliferation and apoptosis.

IF 3.4 4区 医学 Q2 RHEUMATOLOGY Clinical and experimental rheumatology Pub Date : 2024-11-01 Epub Date: 2024-06-19 DOI:10.55563/clinexprheumatol/mh1d4j
Kefen Wang, Yiheng Jiang, Jianzhao Zhai, Pin Zhang, Yuxiang Wu, Haidi Chen, Huapin Zheng, Yang He, Cheng Deng, Yongkang Wu
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Abstract

Objectives: To investigate the expression and function of WNT16, a member of the WNT family protein, in the context of systemic lupus erythematosus (SLE).

Methods: WNT16 expression was assessed in peripheral blood mononuclear cells (PBMCs) from 35 SLE patients and 25 healthy individuals using quantitative polymerase chain reaction. Additionally, serum WNT16 protein levels were quantified via enzyme-linked immunosorbent assay in 162 SLE patients, 96 healthy controls (HC), and disease controls comprised 154 individuals with rheumatoid arthritis (RA) and Sjögren's syndrome (SS). We investigated the associations between WNT16 protein levels and clinical manifestations, laboratory indices, and disease activity in SLE patients. Receiver operating characteristic (ROC) curve analysis was employed to evaluate the diagnostic potential of serum WNT16 for SLE. Furthermore, we performed a knockdown assay on Jeko-1 cells and assessed cell proliferation and apoptosis using Cell Counting Kit-8 and flow cytometry.

Results: WNT16 mRNA in SLE patients' PBMCs were significantly lower than those in HC. Furthermore, serum WNT16 in SLE patients were markedly reduced compared to HC, RA, and SS cohorts. ROC curve analysis indicated that plasma WNT16 levels could serve as a potential biomarker for SLE identification (AUC=0.809, SLE vs. HC; AUC=0.760, SLE vs. RA; AUC=0.710, SLE vs. SS). Notably, a weak positive correlation was observed between WNT16 protein and both alkaline phosphatase and lymphocyte percentages. Conversely, a weak negative correlation existed between WNT16 and low-density lipoprotein, neutrophil percentage, and the incidence of pleurisy and disease activity. Additionally, our study confirmed that WNT16 knockdown impairs cell proliferation and enhances apoptosis.

Conclusions: Serum WNT16 levels effectively differentiate SLE patients from healthy controls and individuals with other autoimmune disorders. WNT16 serves as a potential biomarker with high sensitivity. The diminished expression of WNT16 in SLE may have a significant role in its pathogenesis through the regulation of cell proliferation and apoptosis.

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WNT16作为系统性红斑狼疮的一种有希望的生物标志物及其在调节细胞增殖和凋亡中的作用。
目的研究系统性红斑狼疮(SLE)中 WNT 家族蛋白 WNT16 的表达和功能:方法: 采用定量聚合酶链反应评估了 35 名系统性红斑狼疮患者和 25 名健康人的外周血单核细胞(PBMC)中 WNT16 的表达。此外,我们还通过酶联免疫吸附试验对 162 名系统性红斑狼疮患者、96 名健康对照组(HC)以及 154 名类风湿性关节炎(RA)和斯约格伦综合征(SS)患者的血清 WNT16 蛋白水平进行了定量分析。我们研究了 WNT16 蛋白水平与系统性红斑狼疮患者的临床表现、实验室指标和疾病活动性之间的关系。我们采用接收者操作特征(ROC)曲线分析来评估血清 WNT16 对系统性红斑狼疮的诊断潜力。此外,我们还对Jeko-1细胞进行了基因敲除试验,并使用细胞计数试剂盒-8和流式细胞术评估了细胞增殖和凋亡情况:结果:系统性红斑狼疮患者 PBMC 中的 WNT16 mRNA 明显低于 HC 患者。此外,与HC、RA和SS队列相比,系统性红斑狼疮患者血清中的WNT16明显降低。ROC 曲线分析表明,血浆 WNT16 水平可作为鉴别系统性红斑狼疮的潜在生物标志物(AUC=0.809,系统性红斑狼疮与 HC;AUC=0.760,系统性红斑狼疮与 RA;AUC=0.710,系统性红斑狼疮与 SS)。值得注意的是,WNT16 蛋白与碱性磷酸酶和淋巴细胞百分比之间存在微弱的正相关性。相反,WNT16 蛋白与低密度脂蛋白、中性粒细胞百分比、胸膜炎发病率和疾病活动性之间存在弱负相关。此外,我们的研究还证实,WNT16基因敲除会影响细胞增殖并促进细胞凋亡:结论:血清WNT16水平能有效地区分系统性红斑狼疮患者与健康对照组和其他自身免疫性疾病患者。WNT16是一种潜在的生物标志物,具有很高的灵敏度。WNT16在系统性红斑狼疮中的表达减少可能通过调节细胞增殖和凋亡在其发病机制中起着重要作用。
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来源期刊
CiteScore
6.10
自引率
18.90%
发文量
377
审稿时长
3-6 weeks
期刊介绍: Clinical and Experimental Rheumatology is a bi-monthly international peer-reviewed journal which has been covering all clinical, experimental and translational aspects of musculoskeletal, arthritic and connective tissue diseases since 1983.
期刊最新文献
Group 3 innate lymphoid cells promotes Th17 cells differentiation in rheumatoid arthritis. Mechanism of neutrophil extracellular traps in the pathogenesis of gout. Osteoarticular x-ray reading by medical students followed by eye-tracking: better understanding for better training. Understanding mechanisms of JAK1 inhibition on synovial fibroblasts using combinatorial approaches of bulk and single cell RNAseq analyses. WNT16 as a promising biomarker for systemic lupus erythematosus and its role in regulating cell proliferation and apoptosis.
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